The aim of this work was the study of the best conditions for obtaining a callus culture from the pulp of Acca sellowiana, and to perform a quali-quantitative analysis of the secondary metabolites yielded by the in vitro callus culture. To this end, callus was induced on both Murashige and Skoog and Gamborg B5 media containing various combinations of growth regulators. Three previously undescribed ursane-type triterpenoids, 2α,3β,6α,23-tetrahydroxy-18α,19α-urs-20-en-28-oic acid, 2α,3β,23-trihydroxy-18α,19α-urs-20-en-28-oic acid and 2α,3β,6β,23-tetrahydroxy-18α,19α-urs-20-en-28-oic acid were isolated from the methanolic extract of A. sellowiana culture and characterized by 1D and 2D NMR experiments. Moreover, the quali-quantitative analysis (ESI-MSn and GC-MS) also showed the presence of β-sitosterol, phloridzin, oleanolic, ursolic, 3β-hydroxy-18α,19α-urs-20-en-28-oic, maslinic, corosolic, 2α,3β-dihydroxy-18α,19α-urs-20-en-28-oic, and tormentic acid.

中文翻译：

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在愈伤组织培养物中，从Acca sellowiana（O. Berg）Burret的果肉中产生新的三萜酸。

这项工作的目的是研究从Acca sellowiana的果肉中获得愈伤组织培养物的最佳条件，并对体外愈伤组织培养物产生的次级代谢产物进行定性分析。为此，在含有多种生长调节剂组合的Murashige，Skoog和Gamborg B5培养基上均诱导出愈伤组织。三种先前未曾描述过的乌烷类三萜，2α，3β，6α，23-四羟基-18α，19α-urs-20-en-28-oicacid，2α，3β，23-三羟基-18α，19α-urs-20-en从蝶sell的甲醇提取物中分离出-28-油酸和2α，3β，6β，23-四羟基-18α，19α-urs-20-en-28-油酸并通过一维和二维核磁共振实验对其进行表征。此外，定性分析（ESI-MSn和GC-MS）还显示存在β-谷固醇，phloridzin，齐墩果酸，熊醛，3β-羟基-18α，