当前位置:
X-MOL 学术
›
Anal. Bioanal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Hexagonal cobalt oxyhydroxide nanoflakes/reduced graphene oxide for hydrogen peroxide detection in biological samples
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-10-11 , DOI: 10.1007/s00216-018-1370-6 Xiaoqing Cui , Hong Zhao , Zengxi Li
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-10-11 , DOI: 10.1007/s00216-018-1370-6 Xiaoqing Cui , Hong Zhao , Zengxi Li
Abnormal concentration of hydrogen peroxide (H2O2) in blood plasma and cells may lead to several diseases. Thus, it is important to develop a selective and sensitive method to monitor H2O2. In the present work, a novel nonenzymatic H2O2-sensing platform based on cobalt oxyhydroxide (CoOOH)/reduced graphene oxide (RGO) nanocomposite was fabricated. CoOOH nanoflakes were firstly synthesized via soft chemistry routes and then assembled on the surface of RGO. A series of characterizations by X-ray diffraction, X-ray photoelectron spectroscopy, and transmission electron microscopy demonstrated that hexagonal CoOOH nanoflakes were well distributed on the surface of RGO. The nanocomposite exhibited excellent electrochemical performance for H2O2 detection. Two linear ranges of 6–200 μM and 200–1500 μM were obtained, and the detection limit was 0.01 μM (signal-to-noise ratio was 3). The good performance was attributed to more exposed catalytic active sites of CoOOH nanoflakes compared with zero-dimensional nanoparticles and outstanding conductivity of RGO as well as their synergistic effect. Moreover, the nanocomposite was used to detect H2O2 from human serum and HeLa cells with satisfactory results.
中文翻译:
用于生物样品中过氧化氢检测的六方羟基氧化钴纳米薄片/还原的氧化石墨烯
血浆和细胞中过氧化氢(H 2 O 2)的浓度异常可能导致多种疾病。因此,重要的是开发一种选择性和灵敏的方法来监测H 2 O 2。在本工作中,一种新型的非酶H 2 O 2制备了基于羟基氧化钴(CoOOH)/氧化石墨烯(RGO)纳米复合材料的传感平台。首先通过软化学路线合成了CoOOH纳米薄片,然后将其组装在RGO的表面上。通过X射线衍射,X射线光电子能谱和透射电子显微镜进行的一系列表征表明,六角形的CoOOH纳米薄片很好地分布在RGO的表面上。纳米复合材料对H 2 O 2表现出优异的电化学性能检测。获得了6-200μM和200-1500μM的两个线性范围,检出限为0.01μM(信噪比为3)。良好的性能归因于与零维纳米颗粒相比,CoOOH纳米薄片的更多暴露的催化活性位点,RGO的出色电导率以及它们的协同效应。此外,该纳米复合材料用于检测人血清和HeLa细胞中的H 2 O 2,结果令人满意。
更新日期:2018-10-11
中文翻译:
用于生物样品中过氧化氢检测的六方羟基氧化钴纳米薄片/还原的氧化石墨烯
血浆和细胞中过氧化氢(H 2 O 2)的浓度异常可能导致多种疾病。因此,重要的是开发一种选择性和灵敏的方法来监测H 2 O 2。在本工作中,一种新型的非酶H 2 O 2制备了基于羟基氧化钴(CoOOH)/氧化石墨烯(RGO)纳米复合材料的传感平台。首先通过软化学路线合成了CoOOH纳米薄片,然后将其组装在RGO的表面上。通过X射线衍射,X射线光电子能谱和透射电子显微镜进行的一系列表征表明,六角形的CoOOH纳米薄片很好地分布在RGO的表面上。纳米复合材料对H 2 O 2表现出优异的电化学性能检测。获得了6-200μM和200-1500μM的两个线性范围,检出限为0.01μM(信噪比为3)。良好的性能归因于与零维纳米颗粒相比,CoOOH纳米薄片的更多暴露的催化活性位点,RGO的出色电导率以及它们的协同效应。此外,该纳米复合材料用于检测人血清和HeLa细胞中的H 2 O 2,结果令人满意。