Thermosensitive microgel particles based on the polymeric network synthesized from N‐isopropylacrylamide, sodium acrylate and N,N′‐bisacryloylcystine have been functionalized with aminoferrocene, a mediatior, and a model enzyme‐glucose oxidase (GOx). The product was very stable. A thin coating of that material has been successfully attached to the glassy carbon electrode surface to form a sensing layer. A temperature increase to a value higher than the volume phase transition temperature led to a substantial increase in the amperometric signal. That behavior was well switchable. The amperometric signal was linearly dependent on glucose concentration in the physiological concentration range. It appeared that the synthesized and functionalized microgel was a kind of microreactor and might be very useful for the construction of the platforms that can enhance the activity of enzymes in biosensors.

中文翻译：

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电活性，介导和热敏微凝胶，可用于共价捕获酶并形成生物传感器中的传感层。

由N-异丙基丙烯酰胺，丙烯酸钠和N，N'合成的基于聚合物网络的热敏微凝胶颗粒-双丙烯酰基胱氨酸已通过氨基二茂铁，一种中间物和模型酶-葡萄糖氧化酶（GOx）进行了功能化。产品非常稳定。该材料的薄涂层已成功附着在玻璃碳电极表面上，以形成传感层。温度升高到高于体积相变温度的值导致电流表信号显着增加。这种行为可以很好地切换。安培信号在生理浓度范围内线性依赖于葡萄糖浓度。似乎合成和功能化的微凝胶是一种微反应器，对于构建可增强生物传感器中酶活性的平台可能非常有用。