A label-free proteomics method was used to explore the effects of differentially expressed proteins on the tenderness of yak rumen smooth muscle during postmortem storage (0, 3 and 7 days) at 3 ± 1 °C. The tenderness improved significantly during storage. A total of 212 differentially expressed proteins were identified by the following comparisons: Day 3 vs.0, day 7 vs.0, and day 7 vs.3. Twenty-eight proteins were correlated with the WBSF of yak rumen smooth muscle. Calpastatin, ADP/ATP translocase 1, zyxin, LMOD1 protein, tropomyosin α-3 chain, thrombospondin-4 and UQCRC1 protein are highly related to smooth muscle tenderness, and thus, they are candidates indicators of yak rumen smooth muscle tenderness during storage. Furthermore, bioinformatics analyses revealed that the identified proteins were related to focal adhesion, vascular smooth muscle contraction, cardiac muscle contraction and necroptosis. The present results could provide proteomic insights into changes in yak rumen smooth muscle tenderness during storage and may be a valuable resource for future investigations

使用无标记蛋白质组学方法探讨在3±1°C的死后储存（0、3和7天）过程中差异表达的蛋白质对牛瘤胃平滑肌压痛的影响。贮藏期间的嫩度明显改善。通过以下比较鉴定出总共212种差异表达的蛋白质：第3天vs.0，第7天vs.0和第7天vs.3。28种蛋白质与牛瘤胃平滑肌的WBSF相关。钙蛋白酶抑制素，ADP / ATP转运酶1，zyxin，LMOD1蛋白，原肌球蛋白α-3链，血小板反应蛋白4和UQCRC1蛋白与平滑肌压痛高度相关，因此，它们是during牛瘤胃平滑肌压痛的候选指标。此外，生物信息学分析表明，鉴定出的蛋白质与粘着斑有关，血管平滑肌收缩，心肌收缩和坏死病。目前的结果可以提供蛋白质组学的见解，以了解storage牛瘤胃平滑肌在储存过程中的压痛变化，并且可能是未来研究的宝贵资源