This report presents an integrated biorefinery concept in which wheat straw hydrolysate was treated with co-cultures of osmotolerant thermophilic bacterial strains, Caldicellulosiruptor saccharolyticus and C. owensensis to obtain hydrogen, while the liquid effluent containing acetate and residual glucose was used as feed for polyhydroxybutyrate (PHB) production by Ralstonia eutropha. The Caldicellulosiruptor spp. co-culture consumed 10.8 g/L of pretreated straw sugars, glucose and xylose, producing 134 mmol H₂/L. PHB accumulation by R. eutropha was first studied in minimal salts medium using acetate with/without glucose as carbon source. Addition of salts promoted cell growth and PHB production in the effluent. Fed-batch cultivation in a nitrogen limited medium with 40% (v/v) aeration resulted in a cell density of 15.1 g/L with PHB content of 80.1% w/w and PHB concentration of 12.1 g/L, while 20% aeration gave a cell density of 11.3 g/L with 83.4% w/w PHB content and 9.4 g/L PHB concentration.

本报告提出了一个综合的生物精炼概念，其中将小麦秸秆水解产物与耐渗透性嗜热细菌菌株Caldicellulosiruptor saccharolyticus和C. owensensis的共培养物一起处理以获得氢气，同时将含有乙酸盐和残留葡萄糖的液体废水用作聚羟基丁酸酯的进料（ PHB）由富营养小球藻（Ralstonia eutropha）生产。该Caldicellulosiruptor属。共培养消耗了10.8 g / L的预处理秸秆糖，葡萄糖和木糖，产生134 mmol H ₂ / L。富营养罗非鱼的PHB积累首先在最小盐度培养基中使用乙酸盐（含/不含葡萄糖）作为碳源进行了研究。盐的添加促进了废水中细胞的生长和PHB的产生。在通气量为40％（v / v）的氮限制培养基中进行分批补料培养，得到的细胞密度为15.1 g / L，PHB含量为80.1％w / w，PHB浓度为12.1 g / L，而通气量为20％得到的细胞密度为11.3 g / L，PHB含量为83.4％w / w，PHB浓度为9.4 g / L。