There are relatively few studies concerning the use of coffee leaves for medicinal purposes and the composition of secondary plant substances. Therefore, we identified and quantitated polyphenolic compounds along with caffeine present in methanol extracts of Coffea arabica leaves from three different regions of Brazil (Ceará, Minas Gerais and São Paulo) by HPLC-ESI-MS. In addition, correlations between polyphenolic content of the coffee leaves and antioxidant assays DPPH, FRAP and ORAC were evaluated. Fifteen compounds belonging to three classes of polyphenols (xanthones, chlorogenic acids and flavonoids) along with the alkaloid caffeine were detected. The mean concentration of total polyphenolic compounds in the leaves of C. arabica, harvested from three different regions of Brazil was quite variable. The highest values were detected in the coffee leaves harvested in Minas Gerais (n = 4) at 40.80(13.00) g/kg (SD), followed by coffee leaves harvested in São Paulo (n = 20) at 24.79(20.19) g/kg, and the lowest in coffee leaves harvested in Ceará (n = 11) in the Northeast of Brazil at 10.30(5.61) g/kg. The three classes of polyphenols, all showed excellent correlations in the antioxidant assays. Coffee leaf tea, appears to be an excellent functional beverage, with its high content of polyphenolic compounds, which may render positive biologic effects, when inbibed as part of the normal human diet.

中文翻译：

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营养化合物：从巴西三个地区的阿拉比卡咖啡树的叶子中鉴定并定量了类固醇，类黄酮，黄嘌呤和咖啡因。

关于用于医疗目的使用咖啡叶和次要植物物质组成的研究相对较少。因此，我们通过HPLC-ESI-MS对巴西三个不同地区（塞阿拉，米纳斯吉拉斯州和圣保罗）的阿拉伯咖啡叶甲醇提取物中存在的多酚化合物以及咖啡因进行了鉴定和定量分析。此外，还评估了咖啡叶中多酚含量与抗氧化剂测定DPPH，FRAP和ORAC之间的相关性。与生物碱咖啡因一起检测到属于三类多酚（氧杂蒽酮，绿原酸和类黄酮）的15种化合物。从巴西三个不同地区收获的阿拉比卡茶叶片中总多酚化合物的平均浓度变化很大。在米纳斯吉拉斯州（n = 4）收获的咖啡叶中以40.80（13.00）g / kg（SD）的最高含量检测到最高值，随后在圣保罗（n = 20）中以24.79（20.19）g / kg的咖啡汁中检测到的最高值。公斤，在巴西东北部塞阿拉州（n = 11）收获的最低咖啡叶中为10.30（5.61）g / kg。三类多酚在抗氧化剂测定中均显示出极好的相关性。茶叶茶似乎是一种极好的功能性饮料，其多酚类化合物含量高，当作为正常人类饮食的一部分摄入时，可能会产生积极的生物学作用。三类多酚在抗氧化剂测定中均显示出极好的相关性。茶叶茶似乎是一种极好的功能性饮料，其多酚类化合物含量高，当作为正常人类饮食的一部分摄入时，可能会产生积极的生物学作用。三类多酚在抗氧化剂测定中均显示出极好的相关性。茶叶茶似乎是一种极好的功能性饮料，其多酚类化合物含量高，当作为正常人类饮食的一部分摄入时，可能会产生积极的生物学作用。