Acrylamidase from Cupriavidus oxalaticus ICTDB921 was immobilized on magnetic nanoparticles (MNPs) for degradation of acrylamide (a group 2A carcinogen and an environmental contaminant) from industrial waste water. Acrylamidase-MNPs were prepared (maximum recovery ∼94%) at optimized process parameters viz. 1.5:1 (v/v) of acetone: crude acrylamidase/5 mM of glutaraldehyde/90 min/1.5:1 of enzyme: MNP ratio. MNPs and acrylamidase-MNPs were characterized by particle size analysis, FTIR, XRD, SEM and vibrating sample magnetometer. Acrylamidase-MNPs showed a shift in optimum pH (8–8.5) and temperature (60–65 °C) with higher pH/thermal stability vis-à-vis free enzyme. A significant increase in kinetic constants, thermal inactivation constants and thermodynamic parameters were noted for acrylamidase-MNPs. A complete degradation of acrylamide ∼2100 mg/L was achieved in industrial waste water under optimized conditions for batch process and the kinetics was best represented by Haldane model. Acrylamidase-MNPs retained >80% of its initial activity after 4 cycles for both pure acrylamide and industrial waste water.

来自草酸铜绿菌ICTDB921的丙烯酰胺酶固定在磁性纳米颗粒（MNP）上，用于从工业废水中降解丙烯酰胺（2A类致癌物和环境污染物）。在优化的工艺参数下制备丙烯酰胺酶-MNP（最大回收率约94％）。1.5：1（v / v）的丙酮：丙烯酰胺粗酶/ 5戊二醛/ 90分钟/1.5:1的酶：MNP比。通过粒度分析，FTIR，XRD，SEM和振动样品磁力计对MNPs和丙烯酰胺酶MNPs进行了表征。丙烯酰胺酶-MNPs在最佳pH（8–8.5）和温度（60–65°C）上均发生了变化，相对于更高的pH /热稳定性游离酶。注意到丙烯酰胺酶-MNP的动力学常数，热失活常数和热力学参数显着增加。在工业废水中，在分批工艺的最佳条件下，丙烯酰胺的完全降解达到2100 mg / L，动力学最好用Haldane模型表示。对于纯丙烯酰胺和工业废水，在4个循环后，丙烯酰胺酶MNP保留了其初始活性的80％以上。