Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used ¹⁹F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAHs). An NAH DCL, constructed from a fluoro-aromatic aldehyde and a small set of hydrazides, was targetted at ecFabH, an essential enzyme in bacterial fatty acid biosynthesis. Our NMR analysis identified a tert-butyl NAH as the best binder which was confirmed by enzymatic assay.

中文翻译：

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蛋白质模板化N-酰基hydr动态组合文库的非侵入式¹⁹ F NMR分析

动态组合化学（DCC）是识别生物靶标配体的强大工具。我们使用¹⁹ F NMR作为原位非侵入性技术来测量N-酰基hydr（NAHs）的动态组合文库（DCL）的组成。由氟代芳族醛和少量酰肼构成的NAH DCL靶向ec FabH，它是细菌脂肪酸生物合成中必不可少的酶。我们的NMR分析确定了叔丁基NAH是最好的粘合剂，这已通过酶法测定得到了证实。