Aqueous and ethanol extracts prepared from leaves of Olea europaea L. were evaluated for in vitro antioxidant and in vivo hypocholesterolemic effect. The result of administration of O. europaea leaf extracts on serum total cholesterol, triglyceride, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) in hypercholesterolaemic mice was evaluated. In addition, rutin and luteolin, reported to occur naturally in O. europaea leaves, were docked against HMG-CoA reductase, the rate-limiting enzyme in cholesterol metabolism. Mice treated with both extracts showed reduced total cholesterol (246.6 and 163.4 mg/dl, for mice groups treated with respective extracts) and LDL (150.16 and 81.28 mg/dl, for mice groups treated with respective extracts) levels as compared to the hypercholesterolaemic group (total cholesterol 253.00 mg/dl and LDL 160.00 mg/dl). Mice treated with aqueous extract (200 mg/kg body weight) showed significantly reduced triglyceride and VLDL levels as compared to the group treated with atorvastatine. HDL level of mice administered with O. europaea aqueous extract was comparable to the atorvastatine-treated group. The ethanol extract of O. europeae leaves was a potent antioxidant (IC₅₀ 69.15 mg/ml, % inhibition 54.98, 82.63 mg ascorbic acid equivalent/g extract, 7.53 mol of Fe²⁺/g extract, and % inhibition 49.71, for the DPPH, β-carotene bleaching, total antioxidant capacity, FRAP, and ferric thiocyanate assays, respectively). Docking studies revealed that rutin showed higher binding affinity with HMG-CoA reductase as compared to luteolin. Data gathered from this study support the development of a prophylactic biomedicine from O. europaea leaves for the management of hypercholesterolemia and atherosclerosis.

评价了从油橄榄叶子制备的水和乙醇提取物的体外抗氧化剂和体内降胆固醇作用。评估了欧洲油菜叶提取物对高胆固醇血症小鼠的血清总胆固醇，甘油三酸酯，高密度脂蛋白（HDL），低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）的施用结果。此外，据报道芦丁和木犀草素天然存在于欧洲油O中。叶片与HMG-CoA还原酶对接，HMG-CoA还原酶是胆固醇代谢中的限速酶。与高胆固醇血症组相比，用两种提取物处理的小鼠的总胆固醇水平（对于分别用提取物处理的小鼠组为246.6和163.4 mg / dl）和LDL（对于分别用提取物处理的小鼠组为150.16和81.28 mg / dl）降低（总胆固醇253.00 mg / dl和LDL 160.00 mg / dl）。与用阿托伐他汀治疗的组相比，用水提取物（200 mg / kg体重）处理的小鼠显示出甘油三酯和VLDL水平明显降低。给予欧罗巴草水提取物的小鼠的HDL水平与阿托伐他汀治疗组相当。欧洲油菜叶片的乙醇提取物是有效的抗氧化剂（IC₅₀ 69.15毫克/毫升，抑制％54.98，82.63毫克抗坏血酸当量/克提取物，7.53摩尔的Fe ²⁺ / g的提取物，和抑制％49.71，对于DPPH，β胡萝卜素漂白，总抗氧化能力，FRAP，与分别测定硫氰酸铁）。对接研究表明，芦丁与木犀草素相比，与HMG-CoA还原酶的结合亲和力更高。从这项研究中收集到的数据支持了从油橄榄叶中开发预防性生物医学以治疗高胆固醇血症和动脉粥样硬化的研究。