A novel strategy for the low-cost, high-yield co-production of xylose and xylooligosaccharides together with no xylose inhibition was developed using a novel heterologous expression of XYN10Ks_480 endo-1,4-β-xylanase with a ricin-type β-trefoil type of domain and XYN11Ks_480 endo-1,4-β-xylanase with a CBM 2 superfamily from the Kitasatospora sp in an actinomycetes expression system. Xylose is the main building block for hemicellulose xylan. Our findings demonstrated high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of the extracted xylan of bagasse, and three types of xylan-based substrates were used to produce xylose and xylooligosaccharides. However, hydrolysis by XYN11Ks_480 produced xylooligosaccharides without xylose formation. This study demonstrated how integrating sodium hypochlorite-extracted xylan and enzymatic hydrolysis could provide an alternative strategy for the generation of XOS from lignocellulosic material.

利用XYN10Ks_480内源-1,4 -β-木聚糖酶与蓖麻毒素型β-三叶草的异源表达，开发了一种低成本，高收率生产木糖和木寡糖且无木糖抑制作用的新策略。结构类型和来自Kitasatospora的具有CBM 2超家族的XYN11Ks_480内切-1,4 -β-木聚糖酶sp在放线菌表达系统中。木糖是半纤维素木聚糖的主要构建基块。我们的发现表明，在提取甘蔗渣的木聚糖水解过程中，XYN10Ks_480具有高水平的表达和催化活性，并且使用三种类型的木聚糖基底物来生产木糖和木寡糖。然而，通过XYN11Ks_480的水解产生了没有木糖形成的木寡糖。这项研究表明，整合次氯酸钠提取的木聚糖和酶促水解如何为木质纤维素材料生成XOS提供一种替代策略。