A novel pretreatment strategy based on combination of microwave and ionic liquid [TBA][OH] was developed for enhancing enzymatic hydrolysis of Eucalyptus sawdust. The sugar yield of pretreated sample achieved 410.67 mg/g in 48 h, which suffered from optimized microwave-assisted [TBA][OH] pretreatment. The work mechanism was illuminated by chemical composition, Fourier transform infrared spectroscopy (FTIR), ¹³C cross polarization/magic-angle spinning solid state NMR (¹³C solid NMR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses. The combined effect of microwave and [TBA][OH] leads to the violent deconstruction of lignin, removal of hemicelluloses, destruction of crystalline region and an eroded, pored and irregular micro-morphology. As a green, relatively inexpensive and high efficient pretreatment, microwave-assisted [TBA][OH] pretreatment has great potential in the field of bio-refinery.

提出了一种基于微波和离子液体[TBA] [OH]结合的新型预处理策略，以增强木屑桉木的酶促水解。预处理样品的糖产量在48小时内达到410.67 mg / g，这是经过优化的微波辅助[TBA] [OH]预处理的结果。通过化学成分，傅立叶变换红外光谱（FTIR），¹³ C交叉极化/魔角旋转固态NMR阐明了工作机理（¹³C NMR），X射线衍射（XRD）和扫描电子显微镜（SEM）分析。微波和[TBA] [OH]的共同作用导致木质素的剧烈分解，半纤维素的去除，晶体区域的破坏以及腐蚀，有孔且不规则的微观形态。微波辅助[TBA] [OH]预处理作为一种绿色，相对便宜且高效的预处理方法，在生物精炼领域具有巨大的潜力。