We investigated in vitro and in vivo antioxidant activity of Lactobacillus paracasei ssp. paracasei YBJ01 (LPSP-YBJ01) isolated and identified from fermented yogurt. Strain LPSP-YBJ01 had stress tolerance against acidity, bile salt, and osmotic pressure. Five in vitro antioxidant assays were used to evaluate antioxidant activity of LPSP-YBJ01, which could scavenge free radicals (2,2-diphenyl-1-picrylhydrazyl and hydroxyl) and superoxide anion in vitro. In addition, strain LPSP-YBJ01 had stronger antilipid peroxidation activity and weak reducing power in vitro. We measured in vivo antioxidant activity of LPSP-YBJ01 in an oxidation mouse model induced by d-galactose injection. Strain LPSP-YBJ01 significantly increased serum superoxide dismutase (SOD), glutathione peroxidase, and total-antioxidant capability, and inhibited generation of malondialdehyde in a dose-dependent manner. In addition, strain LPSP-YBJ01 also increased the hepatic and splenic protein expressions of some antioxidant enzymes such as catalase, Cu/Zn-SOD, and Mn-SOD in mice treated with d-galactose. Thus, LPSP-YBJ01 had antioxidant activity in vitro and in vivo and may be a useful probiotic.

我们调查了体外乳酸杆菌副干酪乳ssp的抗氧化活性。从发酵酸奶中分离并鉴定出副干酪酪YBJ01（LPSP-YBJ01）。菌株LPSP-YBJ01具有抵抗酸度，胆汁盐和渗透压的压力耐受性。使用五种体外抗氧化剂测定法来评估LPSP-YBJ01的抗氧化活性，它可以清除自由基（2,2-二苯基-1-吡啶并肼基和羟基）和超氧阴离子。此外，LPSP-YBJ01菌株在体外具有较强的抗脂质过氧化活性和较弱的还原能力。我们在d诱导的氧化小鼠模型中测量了LPSP-YBJ01的体内抗氧化活性-半乳糖注射。菌株LPSP-YBJ01显着增加了血清超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶和总抗氧化剂的能力，并以剂量​​依赖的方式抑制了丙二醛的产生。此外，LPSP-YBJ01菌株还可以在用d-半乳糖治疗的小鼠中增加某些抗氧化酶（如过氧化氢酶，Cu / Zn-SOD和Mn-SOD）的肝和脾蛋白表达。因此，LPSP-YBJ01在体外和体内均具有抗氧化活性，可能是有用的益生菌。