Recent studies have shown that as members of the reactive sulfur species family and the oxidized forms of H₂S, hydrogen polysulfides (H₂S_n) play important roles in physiological and pathological processes. Therefore, accurate detection of H₂S_n levels in living systems is of great significance. In this study, we report an isophorone-based far-red emitting fluorescent probe (RPHS1) for selective ratiometric sensing and imaging of H₂S_n. RPHS1 shows specific ratiometric response towards H₂S_n with fast reaction rate and low detection limit in aqueous media. Furthermore, confocal fluorescence microscopy experiments demonstrate that RPHS1 can be applied for H₂S_n imaging in living cells.

中文翻译：

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基于异佛尔酮的远红外发射比例荧光探针，用于多硫化物的选择性传感和成像†

最近的研究表明，作为活性硫物种家族和H ₂ S的氧化形式的成员，多硫化氢（H ₂ S _n）在生理和病理过程中起着重要的作用。因此，准确检测生命系统中H ₂ S _n的含量具有重要意义。在这项研究中，我们报告了基于异佛尔酮的远红光发射荧光探针（RPHS1），用于H ₂ S _n的选择性比率传感和成像。RPHS1显示出对H ₂ S _n的特定比例响应反应速度快，在水性介质中的检出限低。此外，共聚焦荧光显微镜实验表明，RPHS1可用于活细胞中的H ₂ S _n成像。