Biological studies on in vitro cell cultures are of fundamental importance to investigate cell response to external stimuli, such as new drugs for the treatment of specific pathologies, or to study communication between electrogenic cells. Although three-dimensional (3D) nanostructures brought tremendous improvements on biosensors used for various biological in vitro studies, including drug delivery and electrical recording, there is still a lack of multifunctional capabilities that could help gain deeper insights in several bio-related research fields. In this work, the electrical recording of large cell ensembles and the intracellular delivery of few selected cells are combined on the same device by integrating microfluidic channels on the bottom of a multi-electrode array decorated with 3D hollow nanostructures. The novel platform allows the recording of intracellular-like action potentials from large ensembles of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC) and from the HL-1 line, while different molecules are selectively delivered into single/few targeted cells. The proposed approach shows high potential for enabling new comprehensive studies that can relate drug effects to network level cell communication processes.

中文翻译：

---

MEA生物传感器中结合的选择性细胞内递送和细胞内记录†

体外细胞培养的生物学研究对于研究细胞对外部刺激的反应（例如用于治疗特定病理的新药）或研究电细胞之间的通讯至关重要。尽管三维（3D）纳米结构为用于各种生物体外的生物传感器带来了巨大的进步包括药物输送和电子记录在内的研究，仍然缺乏可以在一些生物相关研究领域获得更深刻见解的多功能功能。在这项工作中，通过在装饰有3D空心纳米结构的多电极阵列底部整合微流体通道，将大细胞集合体的电记录和少数选定细胞的细胞内递送结合在同一设备上。该新型平台可记录源自人类诱导的多能干细胞（hiPSC）和HL-1系的大型心肌细胞的类细胞内动作电位，同时将不同的分子选择性地递送至单个/少量靶向细胞中。