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Engineering enzymes for noncanonical amino acid synthesis.
Chemical Society Reviews ( IF 46.2 ) Pub Date : 2018-10-03 , DOI: 10.1039/c8cs00665b
Patrick J Almhjell 1 , Christina E Boville , Frances H Arnold
Affiliation  

The standard proteinogenic amino acids grant access to a myriad of chemistries that harmonize to create life. Outside of these twenty canonical protein building blocks are countless noncanonical amino acids (ncAAs), either found in nature or created by man. Interest in ncAAs has grown as research has unveiled their importance as precursors to natural products and pharmaceuticals, biological probes, and more. Despite their broad applications, synthesis of ncAAs remains a challenge, as poor stereoselectivity and low functional-group compatibility stymie effective preparative routes. The use of enzymes has emerged as a versatile approach to prepare ncAAs, and nature's enzymes can be engineered to synthesize ncAAs more efficiently and expand the amino acid alphabet. In this tutorial review, we briefly outline different enzyme engineering strategies and then discuss examples where engineering has generated new 'ncAA synthases' for efficient, environmentally benign production of a wide and growing collection of valuable ncAAs.

中文翻译:

用于非规范氨基酸合成的工程酶。

标准的蛋白质氨基酸可访问多种和谐创造生命的化学物质。在这二十种典型的蛋白质构件之外,有无数的非典型氨基酸(ncAA),它们是在自然界中发现的或由人创造的。随着研究表明其作为天然产物和药物,生物探针等的前体的重要性,人们对ncAA的兴趣与日俱增。尽管其广泛的应用,但是由于不良的立体选择性和低的官能团相容性阻碍了有效的制备路线,因此合成ncAA仍然是一个挑战。酶的使用已成为一种制备ncAA的通用方法,自然界中的酶可以被改造为更有效地合成ncAA并扩展氨基酸字母。在本教程复习中,
更新日期:2018-10-03
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