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Nonenzymatic Polymerization into Long Linear RNA Templated by Liquid Crystal Self-Assembly
ACS Nano ( IF 17.1 ) Pub Date : 2018-10-03 00:00:00 , DOI: 10.1021/acsnano.8b05821
Marco Todisco 1 , Tommaso P. Fraccia 1, 2 , Greg P. Smith 3 , Andrea Corno 1 , Lucas Bethge 4 , Sven Klussmann 4 , Elvezia M. Paraboschi 5 , Rosanna Asselta 5, 6 , Diego Colombo 1 , Giuliano Zanchetta 1 , Noel A. Clark 3 , Tommaso Bellini 1
Affiliation  

Self-synthesizing materials, in which supramolecular structuring enhances the formation of new molecules that participate to the process, represent an intriguing notion to account for the first appearance of biomolecules in an abiotic Earth. We present here a study of the abiotic formation of interchain phosphodiester bonds in solutions of short RNA oligomers in various states of supramolecular arrangement and their reaction kinetics. We found a spectrum of conditions in which RNA oligomers self-assemble and phase separate into highly concentrated ordered fluid liquid crystal (LC) microdomains. We show that such supramolecular state provides a template guiding their ligation into hundred-bases long chains. The quantitative analysis presented here demonstrates that nucleic acid LC boosts the rate of end-to-end ligation and suppresses the formation of the otherwise dominant cyclic oligomers. These results strengthen the concept of supramolecular ordering as an efficient pathway toward the emergence of the RNA World in the primordial Earth.

中文翻译:

非酶聚合成液晶自组装模板化的长线性RNA

自合成材料的超分子结构增强了参与该过程的新分子的形成,代表了一个有趣的概念,说明了生物分子在非生物地球中的首次出现。我们在这里介绍了在超分子排列的各种状态的短RNA寡聚体溶液中链间磷酸二酯键非生物形成的研究及其反应动力学。我们发现了一系列的条件,其中RNA寡聚物自组装并相分离成高度浓缩的有序液晶(LC)微区。我们表明,这种超分子状态提供了指导其连接成一百个碱基的长链的模板。此处提供的定量分析表明,核酸LC可提高端对端连接的速率,并抑制原本占优势的环状低聚物的形成。这些结果加强了超分子有序的概念,将其作为通往原始世界RNA世界出现的有效途径。
更新日期:2018-10-03
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