Leukocyte populations quickly respond to tissue damage, but most leukocyte kinetic studies are not based on multiparameter flow cytometry. We systematically investigated several blood leukocyte populations after controlled tissue damage. 48 patients were assigned to either an anterior or posterolateral total hip arthroplasty. Peripheral blood was collected pre-operatively and at 2 h, 24 h, 48 h, 2 and 6 weeks postoperatively and assessed by flow cytometry for absolute counts of multiple leukocyte populations using standardized EuroFlow protocols. Absolute counts of leukocyte subsets differed significantly between consecutive time points. Neutrophils increased instantly after surgery, while most leukocyte subsets initially decreased, followed by increasing cell counts until 48 h. At two weeks all leukocyte counts were restored to pre-operative counts. Immune cell kinetics upon acute tissue damage exhibit reproducible patterns, which differ between the leukocyte subsets and with "opposite kinetics" among monocyte subsets. Flow cytometric leukocyte monitoring can be used to minimally invasively monitor tissue damage.

中文翻译：

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流式细胞术评估白细胞动力学，以监测组织损伤。

白细胞群体对组织损伤迅速作出反应，但是大多数白细胞动力学研究不是基于多参数流式细胞术。我们系统地研究了组织受控制的损伤后几种血液白细胞的数量。48例患者被分配为前路或后外侧全髋关节置换术。术前和术后2 h，24 h，48 h，2和6周收集外周血，并使用标准EuroFlow方案通过流式细胞仪评估多个白细胞种群的绝对计数。在连续的时间点之间，白细胞亚群的绝对计数显着不同。手术后中性粒细胞立即增加，而大多数白细胞亚群最初减少，随后细胞计数增加直至48 h。在两周时，所有白细胞计数恢复到术前计数。急性组织损伤后的免疫细胞动力学表现出可再现的模式，该模式在白细胞亚群之间有所不同，在单核细胞亚群之间具有“相反的动力学”。流式细胞术白细胞监测可用于微创监测组织损伤。