Snake venoms are complex mixtures mainly composed of proteins and small peptides. Crotoxin is one of the most studied components from Crotalus venoms, but many other components are less known due to their low abundance. The venome of Crotalus durissus terrificus, the most lethal Brazilian snake, was investigated by combining its venom gland transcriptome and proteome to create a holistic database of venom compounds unraveling novel toxins. We constructed a cDNA library from C. d. terrificus venom gland using the Illumina platform and investigated its venom proteome through high resolution liquid chromotography–tandem mass spectrometry. After integrating data from both data sets, more than 30 venom components classes were identified by the transcriptomic analysis and 15 of them were detected in the venom proteome. However, few of them (PLA₂, SVMP, SVSP, and VEGF) were relatively abundant. Furthermore, only seven expressed transcripts contributed to ∼82% and ∼73% of the abundance in the transcriptome and proteome, respectively. Additionally, novel venom proteins are reported, and we highlight the importance of using different databases to perform the data integration and discuss the structure of the venom components-related transcripts identified. Concluding, this research paves the way for novel investigations and discovery of future pharmacological agents or targets in the antivenom therapy.

中文翻译：

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巴西响尾蛇响尾蛇的深度蛇毒：结合其毒腺转录组和毒液蛋白质组的综合方法。

蛇毒是复杂的混合物，主要由蛋白质和小肽组成。Crotoxin是Crotalus毒液中研究最多的成分之一，但由于其丰度低，许多其他成分鲜为人知。的venome南美响尾蛇terrificus，最致命的巴西蛇，通过组合研究其毒腺转录组和蛋白质组创建解开新颖毒液毒素化合物的整体数据库。我们从C. d。构建了一个cDNA文库。了不起的使用Illumina平台制备毒液腺，并通过高分辨率液相色谱-串联质谱法研究其毒液蛋白质组。整合两个数据集的数据后，通过转录组分析确定了30多种毒液成分类别，并在毒液蛋白质组中检测到其中15种。但是，其中很少几个（PLA ₂，SVMP，SVSP和VEGF）相对丰富。此外，只有七个表达的转录本分别贡献了转录组和蛋白质组中约82％和约73％的丰度。此外，报告了新型毒液蛋白，我们强调了使用不同数据库进行数据整合的重要性，并讨论了鉴定出的毒液成分相关转录本的结构。最后，这项研究为抗蛇毒疗法中新颖的研究和发现未来药理学药物或靶标铺平了道路。