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Recovery of chlorophylls from spent biomass of Arthrospira platensis obtained after extraction of phycobiliproteins
Bioresource Technology ( IF 11.4 ) Pub Date : 2018-09-29 , DOI: 10.1016/j.biortech.2018.09.141
Hrishikesh A. Tavanandi , K.S.M.S. Raghavarao

Extraction of chlorophylls has received scant attention or priority over phycobiliproteins from Arthrospira platensis. In fact extraction of chlorophylls from spent biomass (left after extraction of phycobiliproteins which goes as waste or underutilized) on drying, will improve the economics of the overall downstream processing. Ethanol (yield 5.75 mg/g, db), being a food grade solvent, was preferred over acetone and dimethyl sulfoxide in spite of their slightly better yields (5.85 mg/g, db). The best conditions were 100% concentration of ethanol, 1:8 S/L ratio, pH 6, 50 °C temperature and 1 h extraction time. An increase of 125% in yield besides reduction of 83.3% in extraction time (from 6 to 1 h) could be achieved at standardized conditions. Low-Humidity drying was observed to be a possible alternative to freeze drying for drying of spent biomass. Ultrasonication as pre-treatment and ethanol as solvent were found effective for extraction of chlorophylls from dry spent biomass.



中文翻译:

从提取藻胆蛋白后获得的钝顶螺旋藻生物量中回收叶绿素

叶绿素提取已收到很少注意或优先于从藻胆蛋白节旋藻。实际上,在干燥时从废生物质中提取叶绿素(提取藻胆蛋白后留下的废物或浪费利用不足的残留物)将改善整个下游加工的经济性。食品级溶剂乙醇(产率5.75 mg / g,db)比丙酮和二甲基亚砜更可取,尽管它们的产率略高(5.85 mg / g,db)。最佳条件为100%乙醇浓度,1:8 S / L比例,pH 6、50°C温度和1 h提取时间。在标准条件下,除提取时间减少83.3%(从6到1小时)外,产量还可提高125%。观察到低湿度干燥可以替代冷冻干燥来干燥废生物质。

更新日期:2018-09-29
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