In this paper, two simple probes (L1 and L2) have been designed and synthesized for detection of ClO⁻ with large Stokes shifts (about 200 nm). Both probes can react with ClO⁻ in less than one min and display obvious changes in fluorescence, which showing potential ability of detection ClO⁻ in real time. ES-MS analysis was utilized to confirm our design strategy and the possible detection mechanism. Owing to the delicate difference of structure of two probes, L2 has a significant fluorescence ratio response to ClO⁻ and the fluorescence of L1 is quenched. L1 has a longer emission wavelength, better response characteristics and weaker cytotoxicity (IC₅₀ 28.1 μM) as compared with L2. The detection limit of L1 is even as low as 0.09 μM. What's more, it is successfully used in monitoring ClO⁻ real water samples and in living cells.

在本文中，两个简单的探针（L1和L2）被设计和用于检测CLO的合成^-用大的斯托克斯频移（约200纳米）。两种探针可以与CLO反应^-在少于一分钟，并显示在荧光明显的变化，这表示检测CLO的潜在能力^-实时。ES-MS分析被用来确认我们的设计策略和可能的检测机制。由于两个探针的结构的微妙差异，L2具有显著荧光比例响应至C10 ^-和的荧光L1被淬灭。L1与L2相比，具有更长的发射波长，更好的响应特性和更弱的细胞毒性（IC ₅₀ 28.1μM）。L1的检测极限甚至低至0.09μM。更重要的是，它成功地监测CLO使用^-实际水样，并在活细胞中。