Since the mercury ions are particularly toxic and they can cause severe harmful effects even at low concentration, searching for selective and sensitive probe to detect Hg²⁺ is of considerable significance. In this study, Rhodamine-naphthalimide conjugate (NRT) has been developed as a novel lysosome-targeted ratiometric Hg²⁺ probe. NRT exhibited colorimetric and dual-channel turn-on fluorescence responses toward Hg²⁺ and could be used to quantify the ion spiked in river water samples. Besides, the probe could also selectively stain lysosomes and sensitively visualize mercury ions in living cells by dual-channel imaging.

中文翻译：

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基于FRET的双通道开启荧光探针，用于检测活细胞中的Hg ²⁺

由于汞离子特别有毒，即使在低浓度下也可能造成严重的有害影响，因此寻找选择性和灵敏的探针检测Hg ²⁺具有十分重要的意义。在这项研究中，若丹明-萘二甲酰亚胺共轭物（NRT）已开发为靶向溶酶体的新型比例Hg ²⁺探针。NRT表现出对Hg ^2+的比色和双通道开启荧光响应，可用于定量测定河水样品中加标的离子。此外，该探针还可以选择性地对溶酶体染色，并通过双通道成像灵敏地观察活细胞中的汞离子。