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UVA-induced neurotoxicity in Japanese medaka (Oryzias latipes)
Photochemical & Photobiological Sciences ( IF 3.1 ) Pub Date : 2018-09-27 00:00:00 , DOI: 10.1039/c8pp00169c
Alaa El-Din H. Sayed 1, 2, 3, 4, 5 , Hamdy A. M. Soliman 2, 3, 6, 7, 8 , Hiroshi Mitani 9, 10, 11, 12, 13
Affiliation  

Ultraviolet radiation-induced neurodegeneration has been studied in the early stages of development in fish, but not extensively in the adult stage. The present study aimed at investigating the effects of ultraviolet radiation-A (UVA) in adult Japanese medaka (Oryzias latipes). The brain, spinal cord, and retina were examined histopathologically as nervous system target organs. Japanese medaka fish were exposed to 15, 30, and 60 min day−1 UVA for 3 days, and samples were obtained 24 h and 14 days after UVA exposure. Neurohistopathological alterations in brain tissue included vacuoles, blood congestion, degeneration of neuropils, and pyknotic nuclei in neurons. Alterations in the spinal cord included neuronal cell degeneration, reduction in the spinal cord area, and degeneration of Mauthner cells. Retinal tissue showed vacuolation in the nerve fiber layer (NFL), pyknotic nuclei in the ganglion cell layer (GCL), and decreased cell populations particularly in the inner nuclear layer (INL) and GCL. The degree of degeneration was dependent on the duration of UVA exposure. The signs of degeneration decreased gradually and disappeared completely after the 14-day recovery period. In addition, p53-deficient medaka fish were more tolerant than were wild-type (Hd-rR) Japanese medaka. In conclusion, UV radiation induced neurodegeneration in the brain, spinal cord, and retina of adult Japanese medaka (Oryzias latipes) but their normal histological architecture reappeared in these tissues after 14 days.

中文翻译:

在日本青鳉UVA诱导的神经毒性(青鳉

紫外线辐射诱发的神经退行性变已在鱼类发育的早期阶段进行了研究,但在成年阶段并未进行广泛的研究。本研究旨在调查紫外线辐射A(UVA)对成年日本(Oryzias latipes)的影响。组织病理学检查了大脑,脊髓和视网膜,将其作为神经系统的靶器官。日本青鳉鱼暴露于15,30和60分钟天-1UVA持续3天,并在暴露于UVA后24小时和14天获得样品。脑组织的神经组织病理学改变包括液泡,血液充血,神经纤维变性和神经元的缩突核。脊髓的改变包括神经元细胞变性,脊髓面积减少和Mauthner细胞变性。视网膜组织在神经纤维层(NFL)中显示出空泡,在神经节细胞层(GCL)中显示了收敛性核,并且细胞数量减少,尤其是在内核层(INL)和GCL中。变性程度取决于暴露于UVA的持续时间。在14天的恢复期后,变性迹象逐渐减少,并完全消失。此外,p53缺失的鱼比野生型(Hd-rR)日本具有更高的耐受性。青鳉),但他们的正常组织架构重新出现在这些组织后14天。
更新日期:2018-09-27
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