Understanding the degradation mechanisms of silk is essential for improving preventive conservation measures, and developing consolidation materials to restore the degraded fibers. Here, modern and historical silk samples were analyzed with an inclusive set of analytical techniques, in order to investigate the changes in the protein secondary structure that occurred upon natural aging or manufacturing processes. FTIR 2D Imaging-Chemical mapping allowed the non-invasive characterization of fibroin structure, and the obtained information was supported by Optical microscopy (OM), Scanning electron microscopy (SEM) and thermal analysis (Thermogravimetry, TGA, and Differential Scanning Calorimetry, DSC). Based on the acquired data, a different degradation stage was proposed for each historical sample, highlighting a heterogeneous conservation status.

了解丝绸的降解机理对于改善预防性保护措施以及开发固结材料以恢复降解的纤维至关重要。在这里，现代和历史丝绸样品使用一套全面的分析技术进行了分析，以研究自然老化或制造过程中发生的蛋白质二级结构的变化。FTIR 2D成像-化学作图可以对血纤蛋白结构进行非侵入性表征，获得的信息得到光学显微镜（OM），扫描电子显微镜（SEM）和热分析（热重分析，TGA和差示扫描量热法（DSC）的支持。根据所获得的数据，为每个历史样本提出了一个不同的降解阶段，从而突出了异构保护状态。