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Adaptive evolution of Clostridium butyricum and scale‐Up for high‐Concentration 1,3‐propanediol production
AIChE Journal ( IF 3.7 ) Pub Date : 2018-10-18 , DOI: 10.1002/aic.16425
Ai-Hui Zhang 1 , Xiao-Yan Zhuang 1 , Kai-Nan Chen 1 , Shi-Yang Huang 1 , Chao-Zhen Xu 1 , Bai-Shan Fang 1, 2, 3
Affiliation  

In order to obtain the excellent 1,3‐propanediol (1,3‐PDO) producer from wild‐type Clostridium butyricum, adaptive evolution was carried out to select the strain for fast growth. The most significant change was that fermentation time decreased from 36 h to 20 h after adaptive evolution. Thus, it led to the corresponding volumetric productivity of 1,3‐PDO increasing from 0.97 to 2.14 (g/L·h−1) which increased by 114%. Adaptive evolution was also applied to butyric acid tolerant strain selected based on the fast‐growing one through a simple equipment. 1,3‐PDO concentration increased from 40.28 to 66.23 g/L through fed‐batch fermentation by butyric acid tolerant strain compared with the fast‐growing one. In addition, the endpoint strain was successfully and steadily used in the 50‐L scale fermentation. Thus, adaptive evolution is an excellent strategy which can help us select the fast‐growing strain and reduce the negative effect from substrate and metabolite inhibition. © 2018 American Institute of Chemical Engineers AIChE J, 65: 32–39, 2019

中文翻译:

丁酸梭菌的适应性进化和高浓度1,3-丙二醇生产的规模放大

为了从野生型丁酸梭状芽胞杆菌中获得优异的1,3-丙二醇(1,3-PDO)生产者,进行了适应性进化以选择可快速生长的菌株。最显着的变化是,适应性进化后,发酵时间从36小时减少到20小时。因此,它导致相应的1,3-PDO的容积生产率从0.97增加到2.14(g / L·h -1),增长了114%。适应性进化还应用于通过简单设备快速增长的耐丁酸菌株。与快速生长的丁酸相比,通过丁酸耐受菌株的分批补料发酵,使1,3-PDO的浓度从40.28 g / L增加到66.23 g / L。此外,终点菌株已成功,稳定地用于50-L规模的发酵中。因此,适应性进化是一种极好的策略,可以帮助我们选择快速生长的菌株并减少底物和代谢物抑制的负面影响。©2018美国化学工程师学会AIChE J,65:32–39,2019
更新日期:2018-10-18
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