Zika virus (ZIKV) is a mosquito-borne flavivirus that leads to devastating consequences for fetal development. However, accurate diagnosis of ZIKV is made difficult by the fact that most infected patients are asymptomatic or present with symptoms similar to those of other febrile illnesses. Thus, the development of a simple, accurate, highly sensitive, and reliable method for the biomedical analysis and diagnosis of ZIKV is needed. Herein, a novel ZIKV liquid biopsy system was constructed via a dendritic Ru(bpy)₃²⁺-polymer-amplified electro-chemiluminescence (ECL) strategy. This system accomplished amplification-free analysis of ZIKV using a drop of blood, and simultaneously achieved a high sensitivity of 500 copies and superior specificity. This strategy adopted the humoral biomarker as the diagnostic index, which greatly simplified the analysis process, and established a nondestructive detection mode. Furthermore, the performance index for biomedical analysis of clinical ZIKV samples was investigated, and the results indicated that the dendritic Ru(bpy)₃²⁺-polymer-amplified ECL strategy reliably responded to ZIKV from the body fluid (blood, saliva, and urine). Hence, this system suitably met the strict clinical requirements for ZIKV detection and thus has the potential to serve as a new paradigm for the biomedical analysis and diagnosis of ZIKV.

中文翻译：

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寨卡病毒液体活检：使用一滴血的树突状Ru（bpy）_{3 2} ^+-聚合物扩增的ECL诊断策略

寨卡病毒（ZIKV）是一种蚊子传播的黄病毒，会对胎儿发育造成毁灭性后果。但是，由于大多数感染患者无症状或出现与其他高热疾病相似的症状，因此很难准确诊断ZIKV。因此，需要开发用于ZIKV的生物医学分析和诊断的简单，准确，高度灵敏和可靠的方法。在这里，通过树突状Ru（bpy）₃ ²⁺构建了一种新型的ZIKV液体活检系统。-聚合物放大电化学发光（ECL）策略。该系统使用一滴血即可完成ZIKV的无扩增分析，并同时实现了500拷贝的高灵敏度和卓越的特异性。该策略采用体液生物标志物作为诊断指标，大大简化了分析过程，建立了无损检测模式。此外，研究了临床ZIKV样品的生物医学分析性能指标，结果表明树突状Ru（bpy）₃ ²⁺-聚合物放大的ECL策略可对体液（血液，唾液和尿液）中的ZIKV做出可靠反应。因此，该系统适当地满足了ZIKV检测的严格临床要求，因此具有作为ZIKV的生物医学分析和诊断的新范例的潜力。