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Determination of sulfamonomethoxine in tilapia (Oreochromis niloticus × Oreochromis mossambicus) by liquid chromatography-tandem mass spectrometry and its application pharmacokinetics study
Journal of Food and Drug Analysis ( IF 3.6 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.jfda.2018.08.007
Te-An Kung , Shu-Hui Lee , Wei-Hsien Wang

A precise and reliable analytical method to measure trace levels of sulfamonomethoxine (SMM) and N4-acetyl metabolite in tilapia samples using liquid chromatography-tandem mass spectrometry was developed. Optimized chromatographic separation was performed on C18 reversed-phase columns using gradient elution with methanol and 5 mmol/L of an ammonium acetate aqueous solution (adjusted to pH 3.5 using formic acid). This study investigated the pharmacokinetic properties and tissue distribution of SMM and its major metabolite N4-acetyl sulfamonomethoxine (AC-SMM) in tilapia after a single dose of 100 mg kg-1 body weight of orally administered SMM. Blood and tissues were collected between 0.5 and 192 h with 14 total sampling time points. SMM was rapidly absorbed, and extensively distributed in the bile and liver through systemic circulation. Enterohepatic circulation of SMM was observed in the tilapia body. Acetylation percentages were 45% (blood), 90% (liver), 62% (kidney), 98% (bile), and 52% (muscle). High concentrations of AC-SMM accumulated in the tilapia bile. At 192 h, AC-SMM concentration in the bile remained at 4710 μg kg-1. The ke value of AC-SMM (0.015 h-1) in the blood was lower than that of SMM (0.032 h-1). This study demonstrated effective residue monitoring and determined the pharmacokinetic properties of SMM and AC-SMM in tilapia.

中文翻译:

液相色谱-串联质谱法测定罗非鱼(Oreochromis niloticus × Oreochromis mossambicus)中磺胺间甲氧嘧啶及其应用药代动力学研究

开发了一种使用液相色谱-串联质谱法测量罗非鱼样品中痕量磺胺间甲氧嘧啶 (SMM) 和 N4-乙酰代谢物的精确可靠的分析方法。使用甲醇和 5 mmol/L 乙酸铵水溶液(使用甲酸将 pH 值调节至 3.5)梯度洗脱,在 C18 反相柱上进行优化的色谱分离。本研究调查了单剂量口服 SMM 100 mg kg-1 体重后 SMM 及其主要代谢物 N4-乙酰磺胺间甲氧嘧啶 (AC-SMM) 在罗非鱼体内的药代动力学特性和组织分布。在 0.5 到 192 小时之间收集血液和组织,共有 14 个采样时间点。SMM吸收迅速,通过体循环广泛分布于胆汁和肝脏。在罗非鱼体内观察到SMM的肝肠循环。乙酰化百分比为 45%(血液)、90%(肝脏)、62%(肾脏)、98%(胆汁)和 52%(肌肉)。罗非鱼胆汁中积累了高浓度的 AC-SMM。在 192 h,胆汁中的 AC-SMM 浓度保持在 4710 μg kg-1。血液中AC-SMM(0.015 h-1)的ke值低于SMM(0.032 h-1)。本研究证明了有效的残留监测并确定了 SMM 和 AC-SMM 在罗非鱼中的药代动力学特性。015 h-1) 低于 SMM (0.032 h-1)。本研究证明了有效的残留监测并确定了 SMM 和 AC-SMM 在罗非鱼中的药代动力学特性。015 h-1) 低于 SMM (0.032 h-1)。本研究证明了有效的残留监测并确定了 SMM 和 AC-SMM 在罗非鱼中的药代动力学特性。
更新日期:2019-01-01
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