Bioresource Technology ( IF 11.4 ) Pub Date : 2018-09-26 , DOI: 10.1016/j.biortech.2018.09.125 Wannapawn Watsuntorn , Chalermchai Ruangchainikom , Eldon R. Rene , Piet N.L. Lens , Warawut Chulalaksananukul
In this study, the activities of hydrogen sulphide (H2S) oxidation and nitrate (N-NO3−) reduction by three pure and mixed strains of nitrate-reducing, sulphide oxidizing bacteria (NR-SOB) were determined. Batch experiments were performed at 35 °C and pH 7.0–8.0 with initial H2S concentrations of 650–900 ppmv and N-NO3− concentrations of ∼120 mg/L. The strains MAL 1HM19, TPN 1HM1 and TPN 3HM1 were capable of removing 100% gas-phase H2S. The co-cultures showed better performance for H2S and N-NO3− removal. The mixed NR-SOB strains showed a higher H2S oxidation rate (143 ± 18 ppmv/h), while the highest N-NO3− removal rate (5.5 ± 0 and 5.1 ± 0.6 N-NO3− mg/L·h) was obtained by a mixture of two NR-SOB strains. The 16S rDNA sequence analysis revealed that all strains belonged to the sub-class Alphaproteobacteria and are closely related to Paracoccus sp. (>99%).
中文翻译:
硝酸盐还原,硫化物氧化细菌的纯培养物和混合培养物从合成废水中去除硫化物和硝酸盐的比较
在这项研究中,硫化氢的活动(H 2 S)氧化和硝酸(N-NO 3 - )还原由硝酸盐还原,硫化物氧化细菌(NR-SOB)的3个纯的和混合的菌株进行了测定。批次实验在35与初始ħ执行℃和pH 7.0-8.0 2 650-900 ppm的的硫的浓度v和N-NO 3 -的~120 mg / L的浓度。菌株MAL 1HM19,TPN 1HM1和TPN 3HM1是能够除去100%的气相H的2 S的共培养物显示出用于h更好的性能2 S和N-NO 3 -的去除。混合的NR-SOB菌株显示出更高的H 2小号的氧化速率(143±18 ppm的v /小时),而最高的N-NO 3 -移除速率(5.5±0和5.1±0.6 N-NO 3 - 是由两个NR的混合物获得毫克/ L·h)的-SOB菌株。该16S rDNA序列分析表明,所有菌株属于子类的α-变形菌和密切相关的副球菌属。(> 99%)。