This study was conducted to evaluate the toxic effects and potency of 2-dodecylcyclobutanone (2-dDCB), a unique compound derived from palmitic acid via irradiation. In a series of assays of bacterial reverse-mutation, in vitro chromosomal aberration, and in vivo micronucleus, negative responses were found by the treatment of 2-dDCB comparing vehicle control, dimethyl sulfoxide or corn oil. In the acute oral toxicity test, all of the mice administrated 2-dDCB survived, and there were no clinical and necropsy signs observed at any doses (0, 300, and 2000 mg/kg body weight) during the experimental period of 14 days. These results suggested that 2-dDCB is a relatively non-toxic substance with median lethality dose higher than 2000 mg/kg body weight. Moreover, there were no adverse effects noted in rats orally administrated 2-dDCB everyday via gavage for 28 days, even at the highest dose (2.0 mg/kg body weight/day) tested, which is 1000-times higher than the human daily intake of 2-dDCB estimated through an extreme exposure scenario. Overall, these results indicate that 2-dDCB is not likely to raise any human health concerns and irradiated foods containing palmitic acid can be recognized as safe for human consumption under the current international regulation systems for food irradiation.

进行这项研究以评估2-十二烷基环丁酮（2-dDCB）的毒性作用和功效，2-十二烷基环丁酮是通过照射从棕榈酸衍生而来的独特化合物。在一系列细菌反向突变，体外染色体畸变和体内试验中通过比较载体对照，二甲基亚砜或玉米油，对2-dDCB的处理发现了微核，阴性反应。在急性口服毒性试验中，所有施用2-dDCB的小鼠均存活，并且在14天的实验期内，在任何剂量（0、300和2000 mg / kg体重）下均未观察到临床和尸检迹象。这些结果表明2-dDCB是一种相对无毒的物质，其致死率中位数高于2000 mg / kg体重。此外，即使每天测试最高剂量（2.0 mg / kg体重/天），经灌胃法连续口服2-dDCB的大鼠，也没有观察到不良反应，这是人类每日摄入量的1000倍，即使是最高剂量（2.0 mg / kg体重/天）也是如此。通过极端暴露场景估算的2-dDCB的百分比。全面的，