Nature presents intriguing biological swimmers with innate energy harvesting abilities from their local environments. Use of natural swimmers as cargo delivery agents presents an alternative strategy to transport therapeutics inside the body to locations otherwise difficult to access by traditional delivery strategies. Herein, a biocompatible biohybrid microswimmer powered by a unicellular freshwater green microalga, Chlamydomonas reinhardtii, is reported. Polyelectrolyte‐functionalized magnetic spherical cargoes (1 µm in diameter) are attached to surface of the microalgae via noncovalent interactions without the requirement for any chemical reaction. The 3D swimming motility of the constructed biohybrid algal microswimmers is characterized in the presence and absence of a uniform magnetic fields. In addition, motility of both microalgae and biohybrid algal microswimmers is investigated in various physiologically relevant conditions, including cell culture medium, human tubal fluid, plasma, and blood. Furthermore, it is demonstrated that the algal microswimmers are cytocompatible when co‐cultured with healthy and cancerous cells. Finally, fluorescent isothiocyanate‐dextran (a water‐soluble polysaccharide) molecules are effectively delivered to mammalian cells using the biohybrid algal microswimmers as a proof‐of‐concept active cargo delivery demonstration. The microswimmer design described here presents a new class of biohybrid microswimmers with greater biocompatibility and motility for targeted delivery applications in medicine.

中文翻译：

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由微藻驱动的微泳器实现主动货运

大自然向有趣的生物游泳者展示了其本地环境中固有的能量收集能力。使用天然游泳者作为货物运输代理人提出了一种替代策略，可以将治疗剂体内运输到传统运输策略难以到达的位置。本文中，由单细胞淡水绿色微藻Chlamydomonas reinhardtii提供动力的生物相容性生物混合微游泳器，据报道。聚电解质功能化的磁性球形货物（直径1 µm）通过非共价相互作用与微藻表面相连，无需任何化学反应。所构造的生物混合藻类微游泳器的3D游泳运动的特征在于是否存在均匀的磁场。另外，在各种生理相关条件下，包括细胞培养基，人输卵管液，血浆和血液，对微藻和生物杂交藻微游动器的运动性进行了研究。此外，还证明了与健康和癌细胞共培养时，藻类微游泳器具有细胞相容性。最后，荧光异硫氰酸酯-右旋糖酐（一种水溶性多糖）分子可以通过生物混合藻类微泳器有效地传递到哺乳动物细胞中，作为概念证明，可以有效地进行货物传递。此处描述的微掠器设计提出了一种新型的生物混合微掠器，具有更高的生物相容性和运动性，可用于药物的靶向输送。