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A novel photoelectrochemical immunosensor for prion protein based on CdTe quantum dots and glucose oxidase
Journal of Electroanalytical Chemistry ( IF 4.5 ) Pub Date : 2018-11-01 , DOI: 10.1016/j.jelechem.2018.09.045
Yanmei Li , Leixia Meng , Kang Zou , Xiaohua Zhang , Jinhua Chen

Abstract In this work, a simple and “signal-off” photoelectrochemical (PEC) immunosensor for prion protein detection was developed taking 3‑Mercaptopropionic acid (MPA)-capped CdTe quantum dots as photocathodic materials and support for the immobilization of antibody (Ab 1 ) of prion protein. In the presence of prion protein, the bioconjugates of secondary antibody (Ab 2 )-gold nanoparticles-glucose oxidase (Ab 2 -AuNPs-GOx) was introduced to the electrode surface due to the specific interaction between prion and its antibodies (Ab 1 and Ab 2 ). Under the addition of glucose, GOx catalyzed the oxidation of glucose and the reduction of the dissolved oxygen to in-situ generates hydrogen peroxide which could deteriorate the PEC properties of MPA-CdTe quantum dots. The consumption of the dissolved oxygen and the deterioration of the PEC properties of the photocathodic materials (MPA-CdTe quantum dots) result in the decrease of the photocurrent of the PEC platform. Under the optimal conditions, the developed PEC immunosensor could be used for sensitive assay of prion protein and showed two linear response ranges from 1 pg mL −1 to 50 pg mL −1 and from 50 pg mL −1 to 1000 pg mL −1 with the detection limit of 0.73 pg mL −1 (3σ). Moreover, this method could be easily generalized for the assay of other proteins by altering the related antibodies, implying its promising applications in bioanalysis and disease diagnostics.

中文翻译:

基于CdTe量子点和葡萄糖氧化酶的朊病毒蛋白光电化学免疫传感器

摘要 在这项工作中,以 3-巯基丙酸 (MPA) 封端的 CdTe 量子点作为光阴极材料和用于固定抗体 (Ab 1 ) 朊病毒蛋白。在朊病毒蛋白存在下,由于朊病毒与其抗体(Ab 1 和抗体)之间的特异性相互作用,将二抗 (Ab 2 )-金纳米颗粒-葡萄糖氧化酶 (Ab 2 -AuNPs-GOx) 的生物偶联物引入电极表面。抗体 2)。在葡萄糖的加入下,GOx 催化葡萄糖氧化,溶解氧原位还原生成过氧化氢,这可能会降低 MPA-CdTe 量子点的 PEC 性能。溶解氧的消耗和光电阴极材料(MPA-CdTe量子点)的PEC性能的恶化导致PEC平台光电流的降低。在最佳条件下,开发的 PEC 免疫传感器可用于朊病毒蛋白的灵敏检测,并显示出两个线性响应范围,从 1 pg mL -1 到 50 pg mL -1 和从 50 pg mL -1 到 1000 pg mL -1 与0.73 pg mL -1 (3σ) 的检测限。此外,通过改变相关抗体,这种方法可以很容易地推广到其他蛋白质的检测,这意味着它在生物分析和疾病诊断中的应用前景广阔。开发的 PEC 免疫传感器可用于朊病毒蛋白的灵敏检测,并显示出两个线性响应范围,从 1 pg mL -1 到 50 pg mL -1 和从 50 pg mL -1 到 1000 pg mL -1,检测限为 0.73 pg mL -1 (3σ)。此外,通过改变相关抗体,这种方法可以很容易地推广到其他蛋白质的检测,这意味着它在生物分析和疾病诊断中的应用前景广阔。开发的 PEC 免疫传感器可用于朊病毒蛋白的灵敏检测,并显示出两个线性响应范围,从 1 pg mL -1 到 50 pg mL -1 和从 50 pg mL -1 到 1000 pg mL -1,检测限为 0.73 pg mL -1 (3σ)。此外,通过改变相关抗体,这种方法可以很容易地推广到其他蛋白质的检测,这意味着它在生物分析和疾病诊断中的应用前景广阔。
更新日期:2018-11-01
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