A novel solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC–MS) method was developed to quantify two new fungicide residuals (Y13149, Y12196) in mung bean sprouts. With a stable and biocompatible elcetrospinning nanofiber (polystyrene/[email protected], PS/[email protected]₂) as coating, the SPME fiber was directly inserted into the stem of mung bean sprout to in-situ in-vivo sampling and extraction, followed by GC–MS analysis. Under the optimal conditions, satisfactory average recoveries of 99% and 72% were obtained for Y13149 and Y12196 with the relative standard deviations (RSDs) under 16.3%, indicating good precision and anti-matrix ability of the method. The result also exhibited low detection limit (0.06–0.08 μg·L⁻¹) and wide liner range (0.3–100 μg·L⁻¹) with the correlation coefficients (R²) of 0.9989. The established method was applied successfully to trace the accumulation and distribution of fungicides in mung bean sprouts, and it provides a simple, rapid and reliable quantitative method for food analysis.

开发了一种新颖的固相微萃取-气相色谱-质谱（SPME-GC-MS）方法来定量测定绿豆芽中两个新的杀菌剂残留量（Y13149，Y12196）。用稳定且生物相容的静电纺丝纳米纤维（聚苯乙烯/ [电子邮件保护]，PS / [电子邮件保护] ₂）作为涂层，将SPME纤维直接插入绿豆芽的茎中，进行​​原位体内采样和提取，然后进行GC-MS分析。在最佳条件下，Y13149和Y12196的平均回收率令人满意，分别为99％和72％，相对标准偏差（RSD）在16.3％以下，表明该方法具有良好的精密度和抗基质能力。结果还显示出较低的检出限（0.06-0.08μg·L ^-1）和宽的衬管范围（0.3–100μg·L^-1）的相关系数（R ²）为0.9989。该方法成功应用于绿豆芽中杀菌剂的积累和分布，为食品分析提供了一种简单，快速，可靠的定量方法。