High hydrostatic pressure (HHP) and electrodialysis with ultrafiltration membranes (EDUF) are two efficient technologies used respectively to improve protein enzymatic hydrolysis and recovery of bioactive peptides, but they have never been tested together. Hence, in this study, HHP pre-treatment was performed on defatted flaxseed protein isolate prior to enzymatic hydrolysis and the resulting peptides were separated by EDUF. HHP pretreatment influenced particle size, protein conformation, and degree of hydrolysis. After EDUF separation, peptide fractions (generated after enzymatic hydrolysis of control and pressure-treated protein isolate) recovered in KCl fraction were enriched in arginine and associated with a decrease in systolic blood pressure (SBP) in spontaneously hypertensive rats. Additionally, the final EDUF hydrolysate generated from pretreated protein and the initial EDUF hydrolysate from native protein were also associated with lower SBP. However, only the control KCl fraction obtained from native protein hydrolysate was associated with anti-diabetic activity.

高静水压（HHP）和超滤膜电渗析（EDUF）是分别用于改善蛋白质酶水解和生物活性肽回收的两种有效技术，但从未一起进行测试。因此，在这项研究中，先对脱脂的亚麻籽蛋白分离物进行HHP预处理，然后再进行酶促水解，然后用EDUF分离得到的肽。HHP预处理影响粒径，蛋白质构象和水解度。EDUF分离后，在自发性高血压大鼠中，在KCl馏分中回收的肽馏分（在对照和经压力处理的蛋白质分离物经酶促水解后生成）富含精氨酸，并与收缩压（SBP）降低相关。此外，预处理蛋白质产生的最终EDUF水解产物和天然蛋白质产生的初始EDUF水解产物也与较低的SBP有关。然而，仅从天然蛋白水解产物获得的对照KCl级分与抗糖尿病活性相关。