We engineered an ingenious fluorescent aptasensor for detection of Pseudomonas aeruginosa (P. aeruginosa) according to the DNA hybridization and fluorescence resonance energy transfer. In the absence of target bacteria, 5-carboxyfluorescein-labeled complementary DNA (FAM-cDNA) hybridizes with the partial sequences of aptamer and the fluorescence of FAM can be quenched by graphene oxide quantum dots (GOQDs). Upon the addition of target bacteria, the aptamer as a biorecognition element is bound with P. aeruginosa specifically. FAM-cDNA prefers to hybridize with the aptamer, resulting in the desorption of FAM-cDNA from GOQDs, thus recovering the fluorescence of FAM. The aptasensor shows a wide linear response to P. aeruginosa in the concentration range of 1.28 × 10³–2.00 × 10⁷ cfu/mL with acceptable selectivity. The detection limit is 100 cfu/mL. The whole process can be finished in 2 h. Moreover, the platform is successfully applied to detect P. aeruginosa in drinking water, orange juice, and popsicle samples.

中文翻译：

---

氧化石墨烯量子点辅助荧光荧光传感器的构建，用于食品样品中铜绿假单胞菌的快速检测

我们设计了一种巧妙的荧光适体传感器，用于根据DNA杂交和荧光共振能量转移来检测铜绿假单胞菌（P. aeruginosa）。在没有靶细菌的情况下，5-羧基荧光素标记的互补DNA（FAM-cDNA）与适体的部分序列杂交，并且FAM的荧光可以被氧化石墨烯量子点（GOQD）淬灭。加入靶细菌后，作为生物识别元件的适体与铜绿假单胞菌特异性结合。FAM-cDNA倾向于与适体杂交，导致FAM-cDNA从GOQD上解吸，从而恢复FAM的荧光。适体传感器显示出对铜绿假单胞菌的宽线性响应在1.28×10 ³ –2.00×10 ⁷ cfu / mL的浓度范围内具有可接受的选择性。检出限为100 cfu / mL。整个过程可以在2小时内完成。此外，该平台已成功应用于检测饮用水，橙汁和冰棒样品中的铜绿假单胞菌。