A simple, sensitive and efficient confirmatory method was developed and validated for the determination of 16 coccidiostats in animal tissues and eggs using hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC-MS/MS). The sample preparation consisted of a solid-liquid extraction with ACN and dispersive SPE cleanup with MgSO₄ and C₁₈. Analysis was realized in an Acquity BEH HILIC silica column, in SRM mode. Both positive and negative ionization was performed, using polarity switching. Isocratic elution was used with a mobile phase of ACN: aqueous ammonium formate 1 mM with 0.1% formic acid (80:20, v/v). Method validation was performed in eggs, poultry, bovine, ovine, porcine and rabbit tissue and exceptionally low LODs were achieved, varying from 0.004 μg kg⁻¹ (decoquinate in porcine tissue) to 0.560 μg kg⁻¹ (halofuginone in eggs). The developed methodology was applied in 82 muscle and egg samples through the Greek National Residue Control Plan for coccidiostats.

开发了一种简单，灵敏，有效的确认方法，并通过亲水相互作用液相色谱-串联质谱（HILIC-MS / MS）测定动物组织和卵中的16种抗球虫药的方法得到了验证。样品制备包括使用ACN的固液萃取和使用MgSO ₄和C _18的分散SPE净化。在Acquity BEH HILIC硅胶柱上，以SRM模式进行分析。使用极性切换可进行正电离和负电离。等度洗脱使用ACN流动相：甲酸铵铵水溶液1 mM，含0.1％甲酸（80：20，v / v）。方法验证是在鸡蛋，家禽，牛，绵羊，猪和兔组织中进行的，LOD异常低，为0.004μgkg^-1（猪组织中的去甲奎宁酸盐）至0.560μgkg ^-1（鸡蛋中的氟丁酮）。通过希腊国家抗球虫药残留控制计划，该开发的方法已应用于82个肌肉和卵的样本中。