Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins. However, despite its important role in scientific research and biotechnological applications, a high-quality metabolic network model for metabolic engineering is yet to be developed. Here, we present the comprehensive metabolic network model of E. coli BL21(DE3), named iHK1487, based on the latest genome reannotation and phenome analysis. The metabolic model consists of 1,164 unique metabolites, 2,701 metabolic reactions, and 1,487 genes. The model was validated and improved by comparing the simulation results with phenome data from phenotype microarray tests. Previous transcriptome profile data was incorporated during model reconstruction, and flux prediction was simulated using the model. iHK1487 was simulated to explore the metabolic features of BL21(DE3) such as broad spectrum amino acid utilization and enhanced flux through the upper glycolytic pathway and TCA cycle. iHK1487 will contribute to systematic understanding of cellular physiology and metabolism of E. coli BL21(DE3) and highlight its biotechnological applications.

大肠杆菌BL21（DE3）是一种工业模型微生物，可大量生产生物产品，例如生物燃料，生物精炼厂和重组蛋白。然而，尽管其在科学研究和生物技术应用中起着重要作用，但尚未开发出用于代谢工程的高质量代谢网络模型。在这里，我们提出了E的综合代谢网络模型。大肠杆菌BL21（DE3），名为iHK1487，基于最新的基因组重新注释和表型分析。代谢模型由1,164种独特的代谢产物，2,701种代谢反应和1,487个基因组成。通过将模拟结果与来自表型微阵列测试的表型数据进行比较，对模型进行了验证和改进。在模型重建过程中，将先前的转录组概况数据纳入其中，并使用该模型模拟通量预测。模拟iHK1487来探索BL21（DE3）的代谢特征，例如广谱氨基酸利用和通过上部糖酵解途径和TCA循环的通量增加。iHK1487将有助于系统理解E的细胞生理和代谢。大肠杆菌BL21（DE3）并突出其生物技术应用。