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Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis.
ACS Infectious Diseases ( IF 5.3 ) Pub Date : 2018-09-21 , DOI: 10.1021/acsinfecdis.8b00163
Ryan J Shirey 1, 2 , Daniel Globisch 1, 2 , Lisa M Eubanks 1, 2 , Mark S Hixon 1, 2 , Kim D Janda 1, 2
Affiliation  

The parasitic disease onchocerciasis is the second leading cause of preventable blindness, afflicting more than 18 million people worldwide. Despite an available treatment, ivermectin, and control efforts by the World Health Organization, onchocerciasis remains a burden in many regions. With an estimated 120 million people living in areas at risk of infection, efforts are now shifting from prevention to surveillance and elimination. The lack of a robust, point-of-care diagnostic for an active Onchocerca infection has been a limiting factor in these efforts. Previously, we reported the discovery of the biomarker N-acetyl-tyramine- O-glucuronide (NATOG) in human urine samples and its ability to track treatment progression between medicated patients relative to placebo; we also established its capability to monitor disease burden in a jird model. NATOG is a human-produced metabolite of tyramine, which itself is produced as a nematode neurotransmitter. The ability of NATOG to distinguish between active and past infection overcomes the limitations of antibody biomarkers and PCR methodologies. Lateral flow immunoassay (LFIA) diagnostics offer the versatility and simplicity to be employed in the field and are inexpensive enough to be utilized in large-scale screening efforts. Herein, we report the development and assessment of a NATOG-based urine LFIA for onchocerciasis, which accurately identified 85% of analyzed patient samples ( N = 27).

中文翻译:

用于监测活动性盘尾丝虫病的无创尿液生物标志物侧向血流免疫测定。

盘尾丝虫病的寄生虫病是可预防的失明的第二大主要原因,全世界折磨了超过1800万人。尽管世界卫生组织提供了可用的治疗方法,伊维菌素和控制措施,但在许多地区,盘尾丝虫病仍然是一种负担。估计有1.2亿人生活在有感染风险的地区,目前的工作正在从预防转向监视和消除。缺乏针对活跃的Onchocerca感染的可靠的即时诊断一直是这些努力的限制因素。先前,我们报道了在人类尿液样本中发现生物标记物N-乙酰基酪胺-O-葡糖醛酸(NATOG)以及追踪药物患者相对于安慰剂的治疗进展的能力。我们还建立了其在Jird模型中监控疾病负担的功能。NATOG是人类产生的酪胺代谢产物,其本身以线虫神经递质的形式产生。NATOG区分活动感染和过去感染的能力克服了抗体生物标志物和PCR方法学的局限性。横向流动免疫测定法(LFIA)诊断提供了在该领域中使用的多功能性和简便性,并且价格便宜,足以用于大规模的筛选工作。在本文中,我们报告了针对盘尾丝虫病的基于NATOG的尿液LFIA的开发和评估,该尿液LFIA准确地确定了85%的分析患者样品(N = 27)。横向流动免疫测定法(LFIA)诊断提供了在该领域中使用的多功能性和简便性,并且价格便宜,足以用于大规模的筛选工作。在本文中,我们报告了针对盘尾丝虫病的基于NATOG的尿液LFIA的开发和评估,该尿液LFIA准确地确定了85%的分析患者样品(N = 27)。横向流动免疫测定法(LFIA)诊断提供了在该领域中应用的多功能性和简便性,并且价格便宜,足以用于大规模的筛选工作。在此,我们报告了基于甲壳素的基于NATOG的尿液LFIA的开发和评估,该尿素LFIA准确地鉴定了85%的分析患者样品(N = 27)。
更新日期:2018-08-24
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