Certified LabDiet^® 5K96 Advanced Protocol™ Verified Casein Diet 10 IF (5K96) is a commercial diet low in soy isoflavones developed for use in developmental and reproductive toxicity (DART) studies, especially those designed to detect endocrine disruptors. The objective of this study was to determine the incidences and severities of 5K96-associated renal lesions in control F₀ and F₁ cohorts of rats fed the 5K96 diet. Kidneys from control animals of four DART studies involving Sprague-Dawley rats fed the 5K96 diet, were evaluated microscopically. Mineralization and basophilic tubules were present in high incidence/severity in males and females compared to historical controls fed conventional diets. F₁ cohorts were affected to a far greater degree than F₀ cohorts, and females were affected more than males. Consideration of target tissue and mode of action should be given before automatically incorporating the 5K96 diet into DART study designs, and caution should be exercised when identifying and interpreting renal toxicity in the F₁ cohorts of such studies.

认证LabDiet ^® 5K96高级协议™验证的酪蛋白日粮10 IF（5K96）是大豆商业饮食低异黄酮发育和生殖毒性（DART）的研究，特别是那些设计用于检测内分泌干扰物使用而开发的。这项研究的目的是确定在以5K96日粮喂养的对照组F ₀和F ₁组中与5K96相关的肾脏病变的发生率和严重程度。在显微镜下评估了四项DART研究的对照动物的肾脏，其中四项DART研究涉及喂食5K96日粮的Sprague-Dawley大鼠。与传统饮食喂养的传统饮食相比，男性和女性的矿物质化和嗜碱性小管的发生率/严重性较高。˚F ₁与F ₀队列相比，这些队列受到的影响要大得多，而女性受到的影响要大于男性。在将5K96饮食自动纳入DART研究设计之前，应考虑目标组织和作用方式，并且在此类研究的F ₁组中识别和解释肾毒性时应谨慎行事。