The effect of gamma radiation delivered over 24 h on the induction of bystander signals of three earthworm species exposed in vivo was investigated: A. chlorotica, A. caliginosa, and E. tetraedra. Worms were exposed to external gamma irradiation (Co-60 source) for 24 h and samples of head, body, and clitellum were dissected from exposed and control worms and placed in culture medium for 24 h at 19 C. The harvested medium was filtered and assayed for expression of bystander signals using both clonogenic and mitochondrial reporter assays. Different responses were observed in the different species and in the different tissues. A. chlorotica worm-treated reporters show insignificant mitochondrial response for all sections, yet a significant clonogenic reduction in survival for body sections. A. caliginosa worm-treated reporters show a significant mitochondrial response for some sections and insignificant mitochondrial response and insignificant reduction in clonogenic survival for the rest. E. tetraedra worms from a control site show significant evidence of bystander signalling, measured by mitochondrial response in reporter cells, for all sections while those harvested from a contaminated site show insignificant changes in baseline signalling when exposed to the challenge dose. In vivo exposure of earthworm species shows evidence of bystander signalling using two different reporter assays. This effect varied between the different species and tissues. There is also evidence of attenuated bystander signalling in worms harvested from a site contaminated with radiation.

研究了在24小时内传递的伽马辐射对体内暴露的三种earth的旁观者信号的诱导的影响：绿叶假单胞菌，caliginosa拟南芥和四面体大肠埃希菌。将蠕虫暴露于外部γ射线（Co-60辐射源）下24小时，然后从暴露的和控制的蠕虫中解剖出头部，身体和斜纹肌样本，并在19°C的培养基中放置24 h。使用克隆发生和线粒体报告基因检测法检测旁观者信号的表达。在不同的物种和不同的组织中观察到不同的反应。绿藻经蠕虫治疗的记者对所有部位的线粒体反应均微不足道，但对人体部位存活的显着克隆形成作用却明显降低。经加利福尼亚州线虫蠕虫治疗的记者对某些切片显示出明显的线粒体反应，其余部分线粒体反应不明显，克隆形成存活率无明显降低。在所有区域中，对照区域中的四面体蠕虫蠕虫均表现出明显的旁观者信号传导证据，通过线粒体反应在报告细胞中测量，而暴露于激发剂量时，从受污染部位收获的那些则在基线信号传导方面无明显变化。体内two物种的暴露显示了使用两种不同的报告基因检测方法发现旁观者信号的证据。这种效果在不同的物种和组织之间有所不同。也有证据表明，从受辐射污染的地点收获的蠕虫中的旁观者信号减弱。