Heat- and ultrasound-assisted extraction methods were applied to recover anthocyanins from Hibiscus sabdariffa calyces. The extraction variables, time (t), ethanol proportion (S), and temperature (T) or ultrasonic power (P), were combined in a 5-level experimental design and analysed by response surface methodology for process optimization. The delphinidin-3-O-sambubioside (C1) and cyanidin-3-O-sambubioside (C2) levels were monitored by LC-DAD-ESI/MSⁿ and used as response criteria. The developed models were successfully fitted to the experimental data and used to determine optimal extraction conditions. UAE was the most efficient method yielding 51.76 mg C1 + C2/g R under optimal conditions (t = 26.1 min, P = 296.6 W and S = 39.1% ethanol, v/v). The dose-response effects of the solid/liquid ratio on the extraction rate were also determined. The anthocyanin levels herein reported are higher than those found in the literature, which support the potential use of H. sabdariffa as a sustainable source of natural colorants with application in different industrial sectors.

应用热和超声辅助提取方法从芙蓉花中回收花青素。提取变量，时间（t），乙醇比例（S）和温度（T）或超声功率（P）组合在5级实验设计中，并通过响应面分析法进行了工艺优化。通过LC-DAD-ESI / MS ⁿ监测delphinidin-3- O - sambubioside（C 1）和花青素3- O - sambubioside（C 2）的水平并用作响应标准。所开发的模型已成功地拟合到实验数据，并用于确定最佳提取条件。阿联酋是最有效的方法，在最佳条件下（t  = 26.1分钟，P  = 296.6 W，S  = 39.1％乙醇，v / v）可产生51.76 mg C1 + C 2 / gR 。还确定了固/液比对提取速率的剂量反应作用。本文报道的花色苷水平高于文献中发现的水平，这支持潜在地使用s。sabdariffa作为可持续的天然着色剂来源，并应用于不同的工业领域。