A series of ferrocenes which contain dinitrogen-fused pyrazolidinone ring were synthesized from acryloylferrocene (4) and N,N′‑cyclic azomethine imines (3). Novel 5‑aryl‑6‑ferrocenoyltetrahydropyrazolo[1,2‑a]pyrazol‑1(5H)-ones were obtained as mixtures of two diastereoisomers (trans and cis) which were separated and isolated as pure substances. Ortho-substituted N,N′‑cyclic azomethine imines 5-oxo-2-(2,4,6-trimethylbenzylidene)pyrazolidin-2-ium-1-ide (3e) and 2-(2-methoxybenzylidene)-5-oxopyrazolidin-2-ium-1-ide (3f) reacted stereoselectively affording only trans-6-ferrocenoyl-5-mesityltetrahydropyrazolo[1,2-a]pyrazol-1(5H)-one (5e) and 6-ferrocenoyl-5-(2-methoxyphenyl)tetrahydropyrazolo[1,2-a]pyrazol-1(5H)-one (5f). Ferrocenyl derivatives were screened for in vitro antioxidant and antifungal activities and excellent DPPH and ABTS radicals scavenging activity was observed with majority of tested 5‑aryl‑6‑ferrocenoyltetrahydropyrazolo[1,2‑a]pyrazol‑1(5H)-ones. Several tested compounds showed selective scavenging properties neutralizing ABTS^•+ radical cations in contrast to inactivity toward DPPH radicals. Trans-5-aryl-6-ferrocenoyltetrahydropyrazolo[1,2-a]pyrazol-1(5H)-ones 5b, 5c, 5d, 5j as well as cis-6-ferrocenoyl-5-(p-tolyl)tetrahydropyrazolo[1,2-a]pyrazol-1(5H)-one (6d) displayed fungal growth inhibition at low concentration against C. albicans and/or A. brasiliensis. Molecular docking studies revealed that the cis-6-ferrocenoyl-5-(4-nitrophenyl)tetrahydropyrazolo[1,2-a]pyrazol-1(5H)-one (6l) and cis-6-ferrocenoyl-5-(naphthalen-2-yl)tetrahydropyrazolo[1,2-a]pyrazol-1(5H)-one (6n) have potential to become lead molecules in drug discovery process.

由丙烯酰基二茂铁（4）和N，N′-环偶氮甲亚胺（3）合成了一系列含二氮稠合吡唑烷酮环的二茂铁。新的5-芳基-6- ferrocenoyltetrahydropyrazolo [1,2一]吡唑-1-（5- ħ） -酮，得到为两个非对映体（的混合物反式和顺式），其分离并分离为纯物质。邻取代Ñ，Ñ ' -环甲亚胺亚胺5-氧代-2-（2,4,6-三甲基亚苄基）吡唑烷-2-鎓-1- IDE（3E）和2-（2-甲氧基亚苄基）-5- oxopyrazolidin -2-ium-1-ide（3f）进行立体选择性反应，仅提供反式-6-二茂铁基-5-甲磺酰基四氢吡唑并[1,2- a ]吡唑-1（5 H）-one（5e）和6-二茂铁基-5-（2-甲氧基苯基）四氢吡唑并[1,2 -一个]吡唑-1-（5- ħ） -酮（5F）。筛选了二茂铁衍生物的体外抗氧化和抗真菌活性，并观察到大多数测试的5-芳基-6-二茂铁酰基四氢吡唑并[1,2 - a ]吡唑-1（5 H）-ones具有优异的DPPH和ABTS自由基清除活性。几种测试化合物显示出中和ABTS的选择性清除特性^•+与对DPPH自由基无活性相反。反式-5 -芳基-6- ferrocenoyltetrahydropyrazolo [1,2一]吡唑-1-（5- ħ） -酮5B，5C，5D，图5j以及顺式- 6 -二茂铁甲酰- 5 - （p -甲苯基）四氢[ 1,2- a ]吡唑-1（5 H）-1（6d）在低浓度下对C表现出抑制真菌生长的作用。白色和/或A。巴西利亚。分子对接研究表明，顺式-6-二茂铁基-5-（4-硝基苯基）四氢吡唑并[1,2 - a ]吡唑-1（5 H）-one（6l）和顺式-6-二茂铁基-5-（萘） -2-基）四氢吡唑并[1,2- a ]吡唑-1（5 H）-one（6n）具有在药物开发过程中成为先导分子的潜力。