Background

Neuroglobin (Ngb) is highly expressed in the suprachiasmatic nucleus, and can regulate Per1 gene expression. It is still not known whether Ngb also influences Cryptochrome (Cry). Cry is implicated in hypertension and primary aldosteronism (PA) in mice. There is a strong correlation between Obstructive Sleep Apnea (OSA) and PA. We propose to prove that Ngb and Cry play a role in OSA with PA.

Methods

Subjects were recruited consecutively from residents of Jakarta, Indonesia; subjects aged 30–65 years with moderate to severe OSA and hypertension were included in the study. OSA was diagnosed using an unattended type 2 portable monitor (Alice Pdx), hypertension was diagnosed when morning blood pressure exceeded 140/90 mmHg or when taking anti-hypertensive drugs. Serum concentration of aldosterone, renin, Cry1, Cry2 and Ngb protein were determined using ELISA method. Primary aldosteronism (PA) was defined as ARR ≥20.

Results

Forty subjects were recruited, 26 male and 14 female, median age 52.5 years, BMI 27.46 kg/m², and AHI 34.8 times/hour. We found 16 subjects with PA and 24 non PA. Cry1 and Cry2 did not correlate with ARR in PA and non PA groups. Ngb correlated positively with Cry1 (Spearman’s rho = 0.455, p = 0.038) but not Cry2 in PA patients. Cry1 concentration decreased in severe hypoxia.

Conclusions

Ngb correlates with Cry1 in OSA with PA. There is no correlation between Cry1 or Cry2 with PA

中文翻译：

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神经球蛋白与隐花色素 1 在阻塞性睡眠呼吸暂停伴原发性醛固酮增多症中的相关性

背景

神经球蛋白（Ngb）在视交叉上核中高表达，可以调节 Per1 基因的表达。目前尚不清楚 Ngb 是否也会影响 Cryptochrome (Cry)。哭泣与小鼠高血压和原发性醛固酮增多症（PA）有关。阻塞性睡眠呼吸暂停 (OSA) 和 PA 之间存在很强的相关性。我们建议通过 PA 证明 Ngb 和 Cry 在 OSA 中发挥作用。

方法

受试者是从印度尼西亚雅加达的居民中连续招募的；该研究包括年龄 30-65 岁、患有中度至重度 OSA 和高血压的受试者。OSA 的诊断使用无人值守的 2 型便携式监测仪（Alice Pdx），当早晨血压超过 140/90 mmHg 或服用抗高血压药物时诊断为高血压。采用ELISA法测定血清醛固酮、肾素、Cry1、Cry2和Ngb蛋白浓度。原发性醛固酮增多症 (PA) 定义为 ARR ≥ 20。

结果

招募40名受试者，其中男性26名，女性14名，中位年龄52.5岁，BMI 27.46 kg/m ²，AHI 34.8次/小时。我们发现 16 名受试者患有 PA，24 名受试者没有 PA。Cry1 和 Cry2 与 PA 和非 PA 组中的 ARR 不相关。PA 患者中 Ngb 与 Cry1 呈正相关（Spearman's rho = 0.455，p = 0.038），但与 Cry2 不相关。严重缺氧时Cry1浓度下降。

结论

Ngb 与患有 PA 的 OSA 中的 Cry1 相关。Cry1或Cry2与PA之间没有相关性