Site-specific chemical modification of proteins can assist in the study of their function. Furthermore, these methods are essential to develop biologicals for diagnostic and therapeutic use. Standard protein engineering protocols and recombinant expression enable the production of proteins with short peptide tags recognized by enzymes capable of site-specific modification. We report here the application of two enzymes of orthogonal specificity, sortase A and butelase 1, to prepare non-natural C-to-C fusion proteins. Using these enzymes, we further demonstrate site-selective installation of different chemical moieties at two sites in a full-size antibody molecule.

中文翻译：

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IgG 1的一锅双标记和通过分选酶A和Butelase 1的组合制备C-to-C融合蛋白

蛋白质的位点特异性化学修饰可以帮助研究其功能。此外，这些方法对于开发用于诊断和治疗用途的生物是必不可少的。标准的蛋白质工程规程和重组表达能够生产带有短肽标签的蛋白质，这些标签可被能够进行位点特异性修饰的酶识别。我们在这里报告了正交特异性的两种酶，分选酶A和丁酸酶1的应用，以制备非天然的C-C融合蛋白。使用这些酶，我们进一步证明了在全尺寸抗体分子的两个位点上不同化学部分的位点选择性安装。