Glucose oxidase (GOD) is an enzyme widely used in glucose monitoring systems owing to its high specificity towards glucose. However, in our previous work maltose was found to show significant interaction with GOD and based on this observation, a novel microplate-based method was developed to assess α-amylase inhibitory activity (GOD method). Concerns regarding the interaction of GOD with maltose has limited the widespread use of the GOD method in assessing α-amylase activity. The present paper provides answers to concerns regarding the interaction of GOD with maltose using HPLC studies and application of the GOD method in assessing α-amylase activity. According to the results, the newly developed GOD method can be considered as a well-suited method for the determination of α-amylase activity and as an easy method to do kinetic studies compared to other available methods.

葡萄糖氧化酶（GOD）由于对葡萄糖具有高度的特异性，因此是一种广泛用于葡萄糖监测系统的酶。但是，在我们以前的工作中，发现麦芽糖显示出与GOD的显着相互作用，并且基于此观察结果，开发了一种基于微孔板的新型方法来评估α-淀粉酶的抑制活性（GOD方法）。关于GOD与麦芽糖的相互作用的担忧限制了GOD方法在评估α-淀粉酶活性中的广泛使用。本文提供了有关利用HPLC研究GOD与麦芽糖相互作用以及GOD方法在评估α-淀粉酶活性方面的关注的答案。根据结果​​，