AbstractA self-contained polymerase chain reaction (PCR) platform with miniaturized power-system is introduced. It is powered by portable lithium batteries and integrated continuous-flow PCR amplification platform. Generally speaking, traditional commercial thermal cyclers rely on external electric supply and thus they are too big in instrument size. This prevents real-timely and field testing during PCR diagnosis. The authors are introducing a continuous-flow 3D spiral microreactor for DNA amplifications and high-resolution multiplexed targets’ detection by utilizing the polyvinyl chloride (PVC) tubing-polymer to fabricate the microreactor for the first time. The whole setup (that can all be placed in one hand) includes (a) the thermo-cycled control (5.5 cm width, 10 cm length and 11 cm height), (b) the passive continuous-flow control, and (c) the trapezoidal PCR microreactor. The PCR platform can work for 4.5 h continuously. With minimal accessories and operations, the total cost of the self-contained PCR machinery is <20 $, much lower than the mainstream of commercial PCR machinery. By waiving external electric supply, this miniaturized PCR platform is applied to amplify the typical DNA fragments of plasma isolated hepatitis B virus (HBV), influenza virus (H7N9avian influenza) bacterium (Escherichia coli) plasmid and multiplexed targets. The efficiency of the method is 70% of that of commercial thermal cycler (CFX Connect, Bio Rad). The DNA of H7N9avian influenza can be detected in concentrations as low as 103 copies per μL. Graphical abstractBy utilizing the polyvinyl chloride (PVC) tubing-polymer to fabricate the microchip for the first time, this paper introduces a 3D spiral microreactor with a miniaturized power-system supplied by portable AA-batteries, applied in DNA amplifications and high-resolution multiplexed targets’ detection. In this microdevice, we made the machinery portable by waiving the external plugs, which solved the problem of traditional commercial thermal cyclers about large volume and expensive price.

中文翻译：

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具有被动微泵和电池供电加热器的 PCR 微反应器机械，用于临床水平和多重 DNA 目标的热循环扩增

摘要 介绍了一种具有小型化动力系统的独立聚合酶链反应(PCR)平台。它由便携式锂电池和集成的连续流 PCR 扩增平台供电。一般来说，传统的商用热循环仪依赖外部供电，因此仪器体积过大。这会妨碍 PCR 诊断期间的实时和现场测试。作者首次利用聚氯乙烯 (PVC) 管状聚合物制造了一种用于 DNA 扩增和高分辨率多重目标检测的连续流动 3D 螺旋微反应器。整个装置（都可以放在一只手上）包括（a）热循环控制（5.5 厘米宽，10 厘米长和 11 厘米高），（b）被动连续流控制，(c) 梯形 PCR 微反应器。PCR平台可连续工作4.5小时。用最少的配件和操作，自成一体的 PCR 机器的总成本 <20 美元，远低于主流的商用 PCR 机器。通过免除外部电源，这种小型化的 PCR 平台被用于扩增血浆分离的乙型肝炎病毒 (HBV)、流感病毒 (H7N9 禽流感) 细菌 (大肠杆菌) 质粒和多重目标的典型 DNA 片段。该方法的效率是商用热循环仪 (CFX Connect, Bio Rad) 的 70%。H7N9 禽流感 DNA 的检测浓度低至每微升 103 个拷贝。图形摘要首次利用聚氯乙烯（PVC）管状聚合物制造微芯片，本文介绍了一种由便携式 AA 电池供电的具有小型化动力系统的 3D 螺旋微反应器，用于 DNA 扩增和高分辨率多重目标检测。在这个微型设备中，我们通过免除外部插头使机器便携，解决了传统商用热循环仪体积大、价格昂贵的问题。