当前位置: X-MOL 学术J. Agric. Food Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Cyanidin-3-O-β-glucoside, a Natural Polyphenol, Exerts Proapoptotic Effects on Activated Platelets and Enhances Megakaryocytic Proplatelet Formation
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2018-09-18 00:00:00 , DOI: 10.1021/acs.jafc.8b03266
Fuli Ya 1, 2, 3 , Jinju Tian 4 , Qing Li 1, 2, 3 , Liyi Chen 5 , Jing Ren 6 , Yimin Zhao 7 , Jianbo Wan 8 , Wenhua Ling 1, 2, 3 , Yan Yang 2, 3, 7
Affiliation  

This study investigated whether the anthocyanin cyanidin-3-O-β-glucoside (Cy-3-g) could affect platelet apoptosis and proplatelet formation in vitro. Thrombin-stimulated or resting human platelets and Meg-01 megakaryocytes were incubated with Cy-3-g (0, 0.5, 5, or 50 μM). We found that the percentage of the platelet mitochondrial membrane potential treated with 5 and 50 μM Cy-3-g was significantly higher than control (15.50% ± 3.24% and 29.77% ± 4.06% versus 2.76% ± 1.33%, respectively; P < 0.05). Treatment with 5 and 50 μM Cy-3-g significantly increased phosphatidylserine exposure compared with control (40.56% ± 10.53% and 76.62% ± 8.28% versus 15.43% ± 3.93%, respectively; P < 0.05). Moreover, Cy-3-g significantly increased the expression of Bax, Bak, and cytochrome c while markedly decreasing Bcl-xL and Bcl-2 expression as well as stimulating caspase-3, caspase-9, caspase-8, Bid, and gelsolin cleavage in thrombin-activated platelets in a dose-dependent manner (P < 0.05). However, no significant differences were observed in the apoptosis of resting platelets when treated with Cy-3-g (P > 0.05). Furthermore, Cy-3-g significantly (P < 0.05) enhanced cell viability (50 μM versus control, 1.34 ± 0.01 versus 0.35 ± 0.02), the number of colony-forming unit-megakaryocytes (50 μM versus control, 38 ± 3 versus 8 ± 3), CD41 expression (50 μM versus control, 96.80% ± 2.55% versus 25.57% ± 2.86%), DNA ploidy (16N) (50 μM versus control, 19.73% ± 2.34% versus 4.42% ± 1.96%), and proplatelet formation (50 μM versus control, 27.5% ± 3.77% versus 7.67% ± 2.25%) in Meg-01 cells. In conclusion, Cy-3-g promotes activated platelet apoptosis and enhances megakaryocyte proliferation, differentiation, and proplatelet formation in vitro.

中文翻译:

Cyanidin-3-O-β-葡萄糖苷,一种天然多酚,对活化的血小板具有促凋亡作用并增强巨核细胞的血小板形成

这项研究调查了花色苷花青素-3-O-β-葡萄糖苷(Cy-3-g)是否可以在体外影响血小板的凋亡和血小板的形成。将凝血酶刺激的或静止的人血小板和Meg-01巨核细胞与Cy-3-g(0、0.5、5或50μM)孵育。我们发现用5和50μMCy-3-g处理的血小板线粒体膜电位百分比显着高于对照(分别为15.50%±3.24%和29.77%±4.06%对2.76%±1.33%; P < 0.05)。与对照组相比,用5和50μMCy-3-g治疗显着增加了磷脂酰丝氨酸的暴露(分别为40.56%±10.53%和76.62%±8.28%与15.43%±3.93%;P <0.05)。此外,Cy-3-g显着增加了Bax,Bak和细胞色素c的表达同时显着降低凝血酶激活的血小板中Bcl-xL和Bcl-2的表达以及刺激凝血酶激活的血小板中的caspase-3,caspase-9,caspase-8,Bid和凝溶胶蛋白裂解(P <0.05)。然而,当用Cy-3-g处理时,静息血小板的凋亡没有观察到显着差异(P > 0.05)。此外,Cy-3-g显着(P<0.05)增强了细胞活力(相对于对照为50μM,相对于对照为1.34±0.01对0.35±0.02),集落形成单位巨核细胞的数量(相对于对照为50μM,对38±3对8±3为阳性),CD41表达(50μM与对照组相比,96.80%±2.55%与25.57%±2.86%),DNA倍性(16N)(50μM与对照,19.73%±2.34%与4.42%±1.96%)和前血小板形成(50μM与对照,27.5) %±3.77%与Meg-01细胞中的7.67%±2.25%)。总之,Cy-3-g促进了活化的血小板凋亡并增强了巨核细胞的增殖,分化和血小板原形成。
更新日期:2018-09-18
down
wechat
bug