Abstract A short chain peptide is covalently attached to an evaporated gold electrode to form a biocompatible self-assembled monolayer (SAM) surface. The peptide is designed to optimize the interfacial interaction of horse heart cytochrome c (cytc) through the electrostatic interaction of the carboxylate group on the terminal amino acid glutamate and the amine on lysine in cytc. Cytc exhibits quasi-reversible voltammetry and is stable on the time scale of the experiments. Marcus theory is used to calculate the electron transfer rates, which are ~5 times faster for cytc/peptide/Au compared to cytc/11-mercaptoundecanoic acid/Au SAMs of similar chain length. The formal potentials also shift for the cytc/peptide system ~20 mV more positive than cytc adsorbed to 11-mercaptoundecanoic acid-SAM/Au electrodes.

中文翻译：

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肽自组装单层上的细胞色素 c 电化学

摘要 短链肽与蒸发的金电极共价连接，形成生物相容性自组装单层 (SAM) 表面。该肽旨在通过末端氨基酸谷氨酸上的羧基和 cytc 中赖氨酸上的胺的静电相互作用来优化马心细胞色素 c (cytc) 的界面相互作用。Cytc 表现出准可逆伏安法并且在实验的时间尺度上是稳定的。Marcus 理论用于计算电子转移速率，与类似链长的 cytc/11-巯基十一烷酸/Au SAM 相比，cytc/肽/Au 的电子转移速率快约 5 倍。cytc/肽系统的正式电位也比吸附到 11-巯基十一烷酸-SAM/Au 电极上的 cytc 更正约 20 mV。