AbstractAn electrochemiluminescence (ECL) based assay is described for the determination of the endocrine disruptor bisphenol A (BPA). The method is based on the use of carboxylated graphitic carbon nitride (C-g-C3N4) carrying an immobilized aptamer against BPA. In the presence of BPA, the ECL signal decreases due to ECL energy transfer from excited-state C-g-C3N4 to the BPA oxidation product. Under the optimal conditions, ECL intensity increases linearly in the 0.1 pM to 1 nM BPA concentration range. The detection limit is as low as 30 fM. The assay has excellent sensitivity, outstanding stability and high selectivity. It was applied to the determination of BPA in spiked water samples. Graphical abstractAptamer modified carboxylated graphitic carbon nitride was synthesized and applied in an electrochemiluminescence-based aptasensor for bisphenol A.

中文翻译：

---

基于适配体的羧化石墨氮化碳电化学发光测定双酚A

摘要描述了一种基于电化学发光 (ECL) 的测定，用于测定内分泌干扰物双酚 A (BPA)。该方法基于使用带有针对 BPA 的固定化适体的羧化石墨氮化碳 (Cg-C3N4)。在 BPA 存在的情况下，由于 ECL 能量从激发态 Cg-C3N4 转移到 BPA 氧化产物，ECL 信号降低。在最佳条件下，ECL 强度在 0.1 pM 至 1 nM BPA 浓度范围内线性增加。检测限低至 30 fM。该测定具有出色的灵敏度、出色的稳定性和高选择性。用于测定加标水样中的 BPA。