To achieve efficient one-step production of gluconic acid, cascade reactions of glucose oxidase (GOD) and catalase (CAT) have been advocated in the biocatalysis system. In this work, the methodology of co-immobilization of GOD and CAT was investigated in details for obtaining improved enzyme loading and activity. The maximum adsorption capability of GOD and CAT was 24.18 and 14.33 mg·g⁻¹, respectively. The matching between dimensions of enzymes and hierarchical pore sizes of carriers are critical to the success of immobilization process. The simultaneous self-assembly on glutaraldehyde cross-linked mesoporous carriers exhibited favorable properties in comparison with sequential immobilization of GOD and CAT. The conversion of glucose under adequate air by co-localized GOD&CAT sustained the activity more than 90% after repeated utilization in the production of sodium gluconate and gluconic acid, suggesting that the co-immobilized GOD&CAT could be a promising catalyst for gluconate and gluconic acid production in some chemical and food industries.

为了实现高效一步法生产葡萄糖酸，已经在生物催化系统中提倡了葡萄糖氧化酶（GOD）和过氧化氢酶（CAT）的级联反应。在这项工作中，详细研究了共固定GOD和CAT的方法，以提高酶的负载和活性。GOD和CAT的最大吸附能力为24.18和14.33 mg·g ^-1， 分别。酶的尺寸和载体的分级孔径之间的匹配对于固定化过程的成功至关重要。与顺序固定GOD和CAT相比，在戊二醛交联的介孔载体上的同时自组装表现出良好的性能。在重复利用葡萄糖和葡萄糖酸生产后，通过共定位的GOD＆CAT在充足的空气下转化葡萄糖可使活性保持90％以上，这表明共固定的GOD＆CAT可能是有希望的葡萄糖和葡萄糖酸生产催化剂。在某些化学和食品工业中。