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ARL3 subcellular localization and its suspected role in autophagy
Biochimie ( IF 3.9 ) Pub Date : 2018-09-15 , DOI: 10.1016/j.biochi.2018.09.007
Guanghong Luo , Yangyang Sun , Ruili Feng , Qinping Zhao , Tieqiao Wen

ADP-ribosylation factor-like3 (ARL3) is a member of the ADP-ribosylation factor family of GTP-binding proteins that plays important role in regulating Ciliary trafficking. It ubiquitously expressed in normal tissues and tumor cell lines. However, the location and function of ARL3 in organelles are rarely known. In this study, we explored ARL3 subcellular localization in an all-round way in HEK293T, Neuro-2A and U251 cells by density gradient centrifugation and immunofluorescence. The results showed that ARL3 is expressed in most of organelles, and an iodixonal step gradient was further confirmed that ARL3 is mainly localized to the mitochondria, endosomes, lysosomes, and proteasome. By molecular functional analysis, we observed that ARL3 promotes the aggregation of GFP-LC3, up-regulation of LC3-II/LC3-I and down-regulation of SQSMT1/BECN1, and knocking down of ARL3 inbibits autophagy, which suggested that ARL3 is necessary for autophagy. this study presents a comprehensive evaluation of the subcellular localization for ARL3 and provides important on understanding the functions of ARL3.



中文翻译:

ARL3亚细胞定位及其在自噬中的可能作用

ADP-核糖基化因子样3(ARL3)是GTP结合蛋白的ADP-核糖基化因子家族的成员,在调节纤毛运输中起重要作用。它在正常组织和肿瘤细胞系中普遍表达。但是,ARL3在细胞器中的位置和功能鲜为人知。在这项研究中,我们通过密度梯度离心和免疫荧光法全面探索了ARL3亚细胞在HEK293T,Neuro-2A和U251细胞中的定位。结果表明,ARL3在大多数细胞器中都有表达,并且进一步证实了IIXX的逐步梯度变化,表明ARL3主要位于线粒体,内体,溶酶体和蛋白酶体中。通过分子功能分析,我们观察到ARL3促进GFP-LC3的聚集,LC3-II / LC3-I的上调和SQSMT1 / BECN1的下调,并敲低ARL3的自噬位,这表明ARL3对于自噬是必需的。这项研究提出了对ARL3亚细胞定位的全面评估,并为理解ARL3的功能提供了重要的信息。

更新日期:2018-09-15
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