Controlled self-assembly of DNA has been under intensive focus owing to its potential utility in the nano-gene device, gene therapy, and targeted drug delivery. Herein, high-order nano-network from self-assembly of c-MYC (oncogene c-MYC promoter pu22 sequences) and c-KIT1 (oncogene c-KIT1 promoter pu27 sequences) have successfully achieved G-quadruplex DNA structure when guided by arene ruthenium(II) complex, RAP-11 ([Ru(η⁶-cymene)(p-ClPIP)Cl]Cl, p-ClPIP: 2-(4-Chlorophenyl)imidazole[4,5f][1,10]-phenanthroline). This self-assembled nano-network structure was confirmed by AFM (atomic force microscopy) and AUC (analytical ultracentrifugation), as well as apparently induced CD (circular dichroism) signals at about 350 nm in the CD spectra of corresponding the G-quadruplex DNA. Further studies showed that the tail structure, with RAP-11 inserted into the minor groove of the G-quadruplex DNA and the head of the molecule extruded from the G-quadruplex structure, can promote head-to-head stacking through π-π stacking and play a key role in guiding well-ordered arrangement of G-quadruplexes thereby forming nano-network structure effectively.

由于DNA在纳米基因设备，基因治疗和靶向药物输送中的潜在用途，因此可控的DNA自组装受到广泛关注。在这里，由c-MYC（致癌基因c-MYC启动子pu22序列）和c-KIT1（致癌基因c-KIT1启动子pu27序列）的自组装形成的高阶纳米网络在芳烃的引导下已成功实现了G-四链体DNA结构。钌（II）络合物，RAP-11（的[Ru（η ⁶ -cymene）（p -ClPIP）CL]氯，p -ClPIP：2-（4-氯苯基）咪唑并[4,5 ˚F] [1,10]-菲咯啉）。这种自组装的纳米网络结构已通过AFM（原子力显微镜）和AUC（分析超速离心）以及相应的G-四链体DNA的CD光谱中约350 nm处的明显诱导的CD（圆二色性）信号证实。 。进一步的研究表明，将RAP-11插入G-四链体DNA的小沟中并从G-四链体结构中挤出分子的头部的尾部结构可以通过π-π堆积促进头对头的堆积在指导G-四链体的有序排列从而有效地形成纳米网络结构中起关键作用。